摘要
为了制备并评价大熊猫(Ailuropoda melanoleuca)重组干扰素γ(IFN-γ)聚氰基丙烯酸正丁酯(PBCA)纳米球,本研究利用蛋白质原核表达技术,获得了重组大熊猫IFN-γ蛋白,然后以PBCA为载药材料,采用乳化聚合法制备了大熊猫干扰素γ纳米微球(IFNγ-PBCA-NS),最后借助感染大熊猫流感病毒A/Panda/Sichuan/01/2011(H1N1)的昆明小鼠(Mus musculus)模型,通过灌胃和皮下注射药物初步评价了IFNγ-PBCA-NS的药效。结果表明,大熊猫IFN-γ的蛋白分子量约为33.5 ku,所制备的大熊猫IFNγ-PBCA-NS外观规整,粒径在50~200 nm之间,跨距0.55,大小较均匀,包封率为56.7%,载药量为0.86%。灌胃和注射两种给药途径中,IFNγ-PBCA-NS组小鼠的生命延长率均显著高于IFN-γ组(P<0.05或P<0.01)。显示IFNγ-PBCA-NS在小鼠体内有更好的缓释和抗病毒作用,可为进一步研究制备大熊猫多肽类药物微粒提供参考。
To prepare and investigate the recombinant Interferon-gamma(IFN-γ) nanosphere loaded in polybutylcyanoacrylate(PBCA) in Giant Panda(Ailuropoda melanoleuca),we firstly obtained the recombinant IFN-γ protein through prokaryotic protein expression technology,and then,by choosing PBCA as the drug delivery material,prepared the INF-γ PBCA nanosphere(IFNγ-PBCA-NS) using an emulsion polymerization method.Further more,the Kunming Mice(Mus musculus),which were infected with giant panda influenza virus A/Panda/Sichuan/01/2011(H1N1),were used as models to test the efficacy of IFNγ-PBCA-NS compared to that of IFNγ after intragastric administration and subcutaneous injection of drugs.The results demonstrated that the molecular weight of INF-γ protein was about 33.5 ku,and the IFNγ-PBCA-NS was globular in appearance,with uniform particle size.The diameters ranged from 50 to 200 nm,the span was 0.55,the encapsulation efficiency of the particles was 56.7% and the drug loading was 0.86%.Moreover,the prolonged life rate of mice in the IFNγ-PBCA-NS group was significantly higher than that of the IFNγ group in both intragastric administration and subcutaneous injection approaches(P〈0.05 or P 〈0.01).In summary,IFNγ-PBCA-NS showed better sustained release and antiviral activity in mice,which could provide references for further research on the preparation of the Giant Panda peptide drug microparticles.
出处
《动物学杂志》
CAS
CSCD
北大核心
2017年第2期314-321,共8页
Chinese Journal of Zoology
基金
四川省科技计划项目(No.2012JQ0037)
关键词
大熊猫
干扰素Γ
聚氰基丙烯酸正丁酯
纳米球
Giant Panda
Ailuropoda melanoleuca
Interferon-gamma
Polybutylcyanoacrylate
Nanosphere