黄芪丹参1∶1配伍对缺氧缺血性脑损伤乳鼠脑组织神经细胞凋亡的影响

Effect of 1:1 matched astragalus membranaceus and salvia miltiorrhiza on apoptosis of cerebral nerve cells of neonatal rats with hypoxic-ischemic brain damage

目的通过研究黄芪丹参1∶1配伍对缺氧缺血性脑损伤(HIBD)乳鼠脑组织Caspase-3、Bcl-2及Bax蛋白表达的影响,探讨其对脑组织的保护作用及机制。方法选择生后7 d SD乳鼠77只,随机分为假手术组(28只)、模型组(28只)及中药组(21只),应用免疫组化法观察不同时间点各组乳鼠脑组织Caspase-3、Bcl-2及Bax蛋白的表达情况。结果模型组在造模后各时间点Caspase-3及Bax的含量均显著高于假手术组,差异有统计学意义(P<0.05),模型组在造模2 h后Bcl-2含量显著高于假手术组,差异有统计学意义(P<0.05),在造模1 d、3 d后Bcl-2含量有升高趋势,但差异无统计学意义,造模7 d后,Bcl-2含量有下降趋势;中药组在造模后各时间点Caspase-3、Bax含量均较模型组显著降低,Bcl-2含量显著升高,差异有统计学意义(P<0.05)。结论黄芪丹参1∶1配伍能有效上调HIBD乳鼠脑组织Bcl-2蛋白表达,下调Caspase-3及Bax蛋白表达,从而抑制神经细胞凋亡,减轻缺氧缺血所致的脑组织损伤。

Objective To research the effect of 1 ∶ 1 matched astragalus membranaceus and salvia miltiorrhiza on expressions of Caspase-3 protein,Bcl-2 protein,and Bax protein in cerebral nerve cells of neonatal rats with hypoxic-ischemic brain damage（HIBD）,explore the protective effects on brain tissues and the mechanism.Methods Seventy-seven 7-day-old neonatal SD rats were selected and randomly divided into sham-operation group（28 rats）,HIBD model group（28 rats）,and Chinese medicine treatment group（21 rats）.Immunohistochemical method was used to observe the expressions of Caspase-3,bcl-2,and Bax in brain tissues of neonatal rats in three groups at different time points.Results After establishing HIBD model,the expressions of Caspase-3 and Bax at different time points in HIBD model group were statistically significantly higher than those in sham-operation group（P〈0.05）.At two hours after establishing HIBD model,Bcl-2 expression in HIBD model group was statistically significantly higher than that in sham-operation group（P〈0.05）.At one and three days after establishing HIBD model,Bcl-2 expression in HIBD model group showed increasing trend,but there was statistically significant difference;at seven days after establishing HIBD model,Bcl-2 expression in HIBD model group showed decreasing trend.After establishing HIBD model,the expressions of Caspase-3 and Bax at different time points in Chinese medicine treatment group were statistically significantly lower than those in HIBD model group,Bcl-2 expression was statistically significantly higher than that in HIBD model group（P〈0.05）.Conclusion 1 ∶ 1 matched astragalus membranaceus and salvia miltiorrhiza can significantly upregulate Bcl-2 expression in brain tissues of neonatal rats with HIBD,downregulate the expressions of Caspase-3 and Bax,inhibit apoptosis of nerve cells,and relieve brain damage induced by hypoxia and ischemia.