HPLC法筛查百花定喘制剂中前胡投料

Screening of Peucedanum praeruptorum in Baihua Dingchuan Preparations by HPLC

目的:通过测定白花前胡甲素、白花前胡乙素和紫花前胡苷的含量,建立筛查百花定喘制剂中投料为前胡还是紫花前胡的HPLC方法。方法:采用Symmentry-C18(4.6 mm×250 mm,5μm)色谱柱,流动相:甲醇(A)-0.1%磷酸溶液(B),梯度洗脱,检测波长为321 nm,流速为1.0 m L·min-1。结果:5批药材的标示名称与试验判断结果不一致;47批百花定喘制剂的筛查结果表明,未见紫花前胡投料,前胡存在投料不足的情况。结论:该方法专属性良好,可作为定性筛查白花定喘制剂中投料前胡的方法。

Objective： To develop an HPLC method for the determination of Praeruptorin A, Praeruptorin B and Nodakenin, so as to screen Peucedanum praeruptorum or Peucedanum decursivum in Baihua Dingchuan Preparations. Methods： The separation was performed with Waters Symmentry-C18（4.6 mm×250 mm, 5 μm） column. A gradient elution of methanol and 0.1% phosphoric acid solution were adopted as the mobile phase at a flow rate of 1.0 m L·min-1. The detection wavelength was 321 nm. Results： The 5 batches of medicinal materials were inconsistent with experimental result. In addition, Peucedanum decursivum was not found in 47 batches of Baihua Dingchuan Preparations, but Peucedanum praeruptorum was in shortage. Conclusion： The established method was specific, reliable and simple, which is adoptable for quality control of Peucedanum praeruptorum in Baihua Dingchuan Preparations.