摘要
目的:建立金钮扣药材的定性定量质量控制方法,考察广西不同产地、不同采收时节金钮扣的药材质量。方法:以乙酸丁酯-乙酸乙酯-甲酸-水(11∶3∶1.5∶1.5)为展开剂,采用TLC法对金钮扣进行定性鉴别。用C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,以乙腈-0.4%磷酸溶液(10∶90)为流动相,流速1.0 m L·min-1,检测波长为327 nm,采用HPLC法对金钮扣中的绿原酸进行含量测定。结果:薄层板上斑点清晰,分离度好;绿原酸在2.032~25.40μg·m L-1(r=0.9995)线性关系良好;加样回收率(n=6)为100.9%,RSD为1.2%,所测得的13批样品含量在0.042~0.741 mg·g-1之间。结论:该方法简便、准确,重复性好,可用于壮药金钮扣药材的质量控制。
Objective: To establish a qualitative and quantitative method for quality control of Spilanthes paniculata Wall. ex DC., and investigate the quality of this medicine in Guangxi at different habitats and harvest time. Methods: The TLC method was used for qualitative identification with butyl acetate-ethyl acetate-formic acid-water(11∶3∶1.5∶1.5) being developing agent. The content determination of chlorogenic acid was conducted by HPLC by using a C18 column(4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.4%phosphoric acid(10∶90) at a flow rate of 1.0 m L·min-1, the detection wavelength was 327 nm, and the column temperature was 30℃. Results: The spot of chlorogenic acid could be well identified by TLC. In the system of HPLC, chlorogenic acid showed good linear relationship in the range of 2.032-25.40 μg·m L-1(r=0.9995). The recovery rate was 100.9%(n=6), RSD=1.2%. The contents of the 13 batches of samples were between 0.042 and 0.741 mg·g-1. Conclusion: The method is simple, accurate, reliable and can be used for the quality control of Spilanthes paniculata Wall. ex DC.
出处
《中国药事》
CAS
2017年第3期290-294,共5页
Chinese Pharmaceutical Affairs
关键词
民族药
壮药
金钮扣
绿原酸
TLC
HPLC
national medicine
Zhuang medicine
spilanthes paniculata
chlorogenic acid
TLC
HPLC