同型半胱氨酸对巨噬细胞CD147表达的影响及瑞舒伐他汀的干预作用

Effects of homocysteine on CD147 expression in macrophages and the intervention effect of rosuvastatin

目的探讨同型半胱氨酸对U-937巨噬细胞CD147表达的影响及瑞舒伐他汀的干预作用。方法在佛波酯诱导分化的人U-937巨噬细胞中加入0、50、100及500μmol/L的同型半胱氨酸孵育48 h;半定量RT-PCR检测CD147 mRNA的表达,Western blot检测巨噬细胞CD147蛋白的表达。不同浓度的瑞舒伐他汀和500μmol/L同型半胱氨酸共同干预U-937巨噬细胞,48 h后半定量RT-PCR及Western blot检测CD147受抑制情况,细胞免疫荧光试验检测CD147表达。结果半定量RT-PCR和Western blot结果显示,随同型半胱氨酸浓度增加,U-937巨噬细胞CD147 mRNA及蛋白的表达逐渐升高,具有剂量依赖性。不同浓度瑞舒伐他汀和500μmol/L同型半胱氨酸共同处理U-937巨噬细胞,随瑞舒伐他汀浓度增加,U-937巨噬细胞CD147 mRNA及蛋白的表达逐渐受抑制,呈一定剂量依赖性。细胞免疫荧光证实同型半胱氨酸促进CD147表达增加,而瑞舒伐他汀能抑制其表达。结论同型半胱氨酸能上调U-937巨噬细胞CD147的表达,瑞舒伐他汀呈浓度依赖性抑制同型半胱氨酸诱导CD147表达。

Aim To investigate the influence of homocysteine（ Hcy） on CD147 expression in human U-937 macrophages and intervention effect of rosuvastatin. Methods Human U-937 macrophages induced by PMA were treated with 0,50,100 and 500 μmol/L Hcy for 48 hours respectively,CD147 expression was then analyzed using Western blot and RT-PCR. Different concentrations of rosuvastatin（ 10^（-8）~ 10^（-5）mol/L） were added when U-937 macrophages were treated with 500 μmol/L Hcy. CD147 expression was then examined after incubating for 48 h. Cellular immumofluorescence method was used to detect the expression of CD147. Results Hcy significantly increased the expression of CD147 in a dose-dependent manner. Rosuvastatin（ 10^-7- 10^-5mol/L） significantly reversed the expression of Hcy-induced CD147 in U-937 macrophages in a dose-dependent manner. Cellular immumofluorescence demonstrated that the CD147 expression of U-937 macrophages was significantly increased by Hcy compared with control group,rosuvastatin（ 10-5mol/L） cloud reverse the effect. Conclusion Hcy（ 100 - 500 μmol/L） significantly increased the production of CD147 in PMA-induced U-937 macrophages in a dose-dependent manner. Additional extracellular rosuvastatin can decrease the expression of CD147 in PMA-induced U-937 macrophages induced by Hcy.