大鼠肝硬化模型中门静脉压力无创评估的初步探讨

Primary Study on Non-invasive Evaluation of Portal Vein Pressure in Model of Liver Cirrhosis in Rats

背景:门静脉压力测量对评估肝硬化患者的治疗效果和预后具有重要意义。目的:探讨无创评估大鼠肝硬化模型门静脉压力的方法。方法:大鼠腹腔注射硫代乙酰胺制备肝硬化模型。造模完成后使用磁共振TOF序列测量大鼠门静脉直径(PVD)。麻醉后以穿刺插管测压法测定门静脉压力,测定大鼠体质量、肝脏重量、脾脏重量、肝脏体积和脾脏体积,碱水解法检测肝脏羟脯氨酸含量,天狼星红染色测定胶原面积比例。结果:腹腔注射20周后成功制备大鼠肝硬化模型。模型组平均门静脉压力、肝脏重量、肝脏体积、脾脏重量、PVD、肝脏体积与体质量比(LV/BW)、脾脏体积与体质量比(SV/BW)、羟脯氨酸含量、胶原面积比例显著高于对照组(P<0.05),体质量显著降低(P<0.001)。相关性分析发现LV/BW和肝脏胶原面积比例与门静脉压力成线性正相关(P<0.05)。结论:LV/BW以及肝脏胶原面积比例可间接反映门静脉压力,有望为临床无创诊断门静脉高压提供重要手段。

Background：The measurement of portal vein pressure（PVP）is important for the evaluation of therapeutic efficacy and prognosis in patients with liver cirrhosis.Aims：To investigate a non-invasive method for evaluating PVP in model of liver cirrhosis in rats.Methods：Liver cirrhosis model in rats was induced by intraperitoneal injection with thioacetamide.Magnetic resonance imaging with TOF sequence was used to measure portal vein diameter（PVD）.PVP was detected directly by transvenous catheterization of portal vein.Body weight,liver weight,spleen weight,liver volume and spleen volume were determined.The hydroxyproline content in liver was determined by alkaline hydrolysis assay,proportion of collagen area in liver was detected by Sirius red staining.Results：Liver cirrhosis model in rats was successfully established after intraperitoneal injection for 20 weeks.Compared with control group,mean PVP,liver weight,liver volume,spleen weight,PVD,liver volume/body weight（LV/BW）ratio,spleen volume/body weight（SV/BW）ratio,hydroxyproline content and proportion of collagen area were significantly increased in model group（P〈0.05）,and body weight was significantly decreased（P〈0.001）.PVP was positively correlated with LV/BW ratio and proportion of collagen area（P〈0.05）.Conclusions：LV/BW and proportion of collagen area can indirectly reflect the PVP,and may provide a noninvasive approach for evaluation of portal hypertension.