NIRS结合TQ软件对氯化铵掺假牛奶定量分析

Quantitative Analysis of Ammonium Chloride Adulterated Milk by Near Infrared Spectroscopy Combined with TQ Software

[目的]应用近红外光谱法建立氯化铵掺假牛奶定量分析模型。[方法]氯化铵是提高牛奶中含氮量的典型掺假物质,样品直接使用近红外光谱仪采用漫反射和三氯乙酸预处理后使用透射模块分别扫描并建立定量分析模型,并对模型进行验证。[结果]建立了漫反射氯化铵含量定量分析模型和透射氯化铵含量定量分析模型,后者模型更加准确可靠,均方根校正标准差(RMSEC)、相关系数(R2)、均方根预测标准差(RMSEP)分别为0.032 4、0.998 4、0.049 8,回收率为107.607 4%。[结论]三氯乙酸预处理后的透射模型更加精确,可以用于牛奶中氯化铵掺假检测,为进一步研究牛奶中其他物质掺假检测提供借鉴。

[ Objective ] Near infrared spectroscopy （NIRS） technology was used for establish quantitative analysis model of ammonium chloride adulterated milk. [ Method] Ammonium chloride is a kind of typical adulterated substance used to increase the nitrogen content of milk. The calibration models were built with infrared diffuse reflection spectrum and the near-infrared transmission spectrum of the samples with a pretreatment with triehloroaeetie acid and then were validated. [ Result ] The root mean square error of cross （ RMSEC ）, the correlation coefficient （ R2 ） and the root mean square error of prediction （RMSEP） of the established model was 0. 032 4, 0. 998 4, and 0. 049 8 respectively. Recovery was 107. 607 4%. [ Conclusion] The overall results showed that the latter calibration model, which was developed with the near-infrared transmission spectrum of the samples with a pretreatment with trichloroaeetie acid, could be more accurate and could be used for rapid analysis of ammonium chloride adulteration in milk.