43.5%吡虫·氟虫腈悬浮种衣剂的高效液相色谱分析

Analysis of Imidacloprid+Fipronil 43.5% FS by HPLC

建立一种高效液相色谱法,对43.5%吡虫·氟虫腈悬浮种衣剂中有效成分进行分离和定量分析。方法使用HYPERSIL BDS-C18色谱柱和紫外可变波长检测器,以甲醇+水为流动相,在268nm波长下采用外标法定量。氟虫腈的标准偏差为0.045 3,变异系数为0.32%,回收率为98.3%~100.3%;吡虫啉的标准偏差为0.107 9,变异系数为0.37%,回收率为99.6%~100.2%。

A HPLC method for the determination of active ingredients in imidacloprid ＋ fipronil 43.5% FS was established. The simulataneous determination of fipronil and imidacloprid in the samples was conducted with HYPERSIL BDS-C18 column and UV-absorbance detector, while the mobile phase was a mixture of methanol and water, and the determine wavelength was 268 nm. The results showed that the standard deviation of fipronil was 0.045 3, the coefficient of variation was 0.32%, and the recoveries were 98.3%-100.3%. The standard deviation of imidacloprid was 0.107 9, the coefficient of variation was 0.37%, and the recoveries were 99.6%-100.2%.