MRP-1、MDR1、GST-π mRNA在CD133^+肿瘤干细胞中的表达分析

Analysis of the expression of MRP-1,MDR1 and GST-π mRNA in CD133^+ tumor stem cells

目的分离鉴定CD133^+肿瘤干细胞,初步分析MRP-1、MDR1、GST-π在CD133^+胶质瘤干细胞多药耐药性中的作用。方法采用胶质瘤U+251细胞系、磁珠分离,培养CD133^+胶质瘤干细胞,RT-PCR技术分析MRP-1、MDR1、GST-π在CD133^+胶质瘤干细胞中的表达。结果培养的胶质母细胞瘤干细胞表达干细胞标记物Nestin,分化后细胞表达GFAP、β-tubulin;MRP-1、MDR1、GST-π在CD133^+胶质瘤干细胞中呈高表达,与CD133-胶质瘤干细胞比较差异有统计学意义(P<0.05)。结论培养、鉴定胶质母细胞瘤肿瘤干细胞、MRP-1、M DR1、GST-π在CD133^+胶质瘤干细胞中呈高表达,为下一步研究奠定基础。

Objective To isolate and identify CD133＋ tumor stem cells, and to preliminary analyze the role of MRP-1, MDR1 and GST-π in multidrug resistance of CD133＋ glioma stem cells. Methods Glioma U251 cell line was applied, and CD133＋ glioma stem cells were isolated by magnetic beads and cultured. RT-PCR was used to analyze the expres- sion of MRP-1, MDR1 and GST-π in CD133＋ glioma stem cells. Results The cultured glioblastoma stem cells ex- pressed stem cell markers Nestin. After differentiation, the cell expressed GFAP and β-tubulin; MRP-1, MDRI and GST-π were highly expressed in CD133＋ glioma stem cells, and there was statistically significant difference compared with CD133-glioma stem cells（P〈0.05）. Conclusion Glioblastoma stem cells are cultured and identified, and MRP-1, MDR1 and GST-π are highly expressed in CD133＋ glioma stem cells, which lays the foundation for further study.