益气化瘀解毒方对急性呼吸窘迫综合征大鼠NF-κB/p38MAPK通路的影响

Impact of Yiqi Huayu Jiedu formula on NF-κB/p38MAPK signal pathway in acute respiratory distress syndrome rats

目的观察益气化瘀解毒方对脂多糖(lipopolysaccharide,LPS)所致大鼠急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)的保护作用并探讨其作用机制。方法 30只SD大鼠随机分为空白组、模型组及益气化瘀解毒方低、中、高剂量组5组,每组6只。尾静脉注射LPS建立ARDS模型。治疗组益气化瘀解毒方灌胃,造模后16小时处死大鼠收集标本,测定大鼠肺系数、肺通透指数、核转录因子-κB p50、IκBα、p38的蛋白表达及光镜下观察肺组织病理学改变。结果益气化瘀解毒方减少肺损伤大鼠肺泡结构破坏、肺水肿及炎性细胞浸润,降低肺系数(P<0.05)与肺通透指数(P<0.01),并在一定程度减少核转录因子-κB p50、丝裂原活化蛋白激酶p38,增加抑制因子IκBα的蛋白表达。结论益气化瘀解毒方对LPS诱导的ARDS有一定的保护作用,其机制与抑制NF-κB/p38MAPK炎性反应信号通路有关。

Objective To observe the impact and mechanism of Yiqi huayu jiedu formula on acute respiratory distress syndrome（ ARDS） rats induced by endotoxin. Methods 30 SD rats were randomized control group,model group,YHJ high,middle,low dose group,6 rats in each group. The ARDS model was established by tail vein injection with lipopolysaccharide. The treatment groups were given corresponding aqueous solution once a day. All groups were sacrificed 16 hours after modeling. Then the lung coefficient,the lung permeability index and the levels of NF-κB p50,IκBα and p38 were measured,and the pathological changes in lung tissues were observed. Results HE staining showed that Yiqi huayu jiedu formula could reduce pulmonary alveolar damage,pulmonary edema and inflammatory cells infiltration. The lung coefficient（ P 〈 0. 05） and lung permeability index（ P 〈 0. 01） were decreased in treatment groups.The protein expressions of NF-κB p50 and p38 were decreased while IκBα was increased in treatment groups. Conclusion Yiqi huayu jiedu formula had prevention effect on endotoxin induced ARDS. It might relate to the inhibition of NF-κB / p38 MAPK inflammatory signal pathway.