高效液相色谱法测定黄芪赤风汤提取物中萜类和色酮类成分的含量

RP-HPLC analysis of terpenoids and chromones in the extractive of Huangqi Chifeng decoction

目的建立HPLC法测定黄芪赤风汤提取物中萜类和色酮类成分的含量。方法采用Venusil MP-C_(18)(4.6×250 mm,5μm)色谱柱;以乙腈(A)-水(B)为流动相进行梯度洗脱(0~45 min,10%→18%A;45~100 min,18%→45%A);检测波长:220 nm;流速:1.0 mL/min;柱温:35℃;LCMS-IT-TOF法指认萜类和色酮类成分,分别以芍药苷和毛蕊异黄酮苷为参比计算两类成分含量。结果芍药苷和毛蕊异黄酮苷的线性范围分别为0.279~6.696μg和0.192~4.608μg(r=0.999 8);平均回收率(n=6)分别为98.83%和98.21%,RSD分别为3.06%和3.24%;经LCMS-IT-TOF法指认萜类成分4个,色酮类成分12个。结论所建立的方法快速、简单、准确、专属性强,精密度好,可用于黄芪赤风汤提取物的质量控制。

Objective To develop an HPLC method for determination of terpenoids and chromones in the extractive of Huangqi chifeng decoction. Methods Venusil MP-C18（ 4. 6 × 250 mm,5 μm） was used to detect. The mobile phase was composed of acetonitrile（ A） and water（ B） with gradient elution（ 0 45 min,10% →18% A; 45 100 min,18% →45% A） and the flow rate was 1. 0 mL/min. The detection wavelength was 220 nm. The column temperature was 35℃. Terpenoids and chromones were identified by LCMS-IT-TOF method,paeoniflorin and calycosin-7-glucoside was regarded as reference to calculate. Results The linear ranges of paeoniflorin and calycosin-7-glucoside were 0. 279 6. 696 μg and 0.192 4. 608 μg（ r = 0. 999 8） respectively. The average recovery was 98. 83% and 98. 21 %（ RSD 〈 3.24%,n = 6） respectively. Four terpenoids and twelve chromones were identified by LCMS-IT-TOF. Conclusion The method is simple,accurate,repeatable and can be used to control the quality of the extractive of Huangqi chifeng decoction.