不同产地蝉花中腺苷、虫草素和麦角甾醇的含量比较

Comparing research on contents of adenosine,cordycepin and ergosterol in cordyceps cicadae

目的分析比较10批不同产地蝉花中腺苷、虫草素和麦角甾醇的含量,为蝉花的质量评价及生产用药的筛选提供方法和基础数据。方法采用高效液相色谱法分别测定不同产地蝉花中腺苷、虫草素和麦角甾醇的含量。结果腺苷以江苏茅山的蝉花含量最高,为44.957μg/g,广西玉林的最低,为11.331μg/g;虫草素以四川宜宾的含量最高,为191.725μg/g,江西九江的蝉花含量最低,为25.437μg/g;各产地麦角甾醇含量差异相对较小,范围为120.024~301.652μg/g。结论不同产地蝉花中的腺苷、虫草素和麦角甾醇的含量差异较大;该方法适用于蝉花中腺苷、虫草素和麦角甾醇的含量测定,为其质量评价提供参考。

Objective The contents of adenosine、cordycepin and ergosterol in cordyceps cicadae from 10 different areas were compared to provide basis for the quality evaluation and utilization of cordyceps cicadae. Methods The high performance liquid chromatography（ HPLC） was used to measure the contents of adenosine、cordycepin and ergosterol in cordyceps cicadae from different areas. Results The highest content of adenosine in cordyceps cicadae was 44. 957 μg / g from Jiangsu,and the lowest was 11. 331μg /g from Guangxi. The highest content of cordycepin was from Sichuan and the content was 191. 725 μg /g,and that from Jiangsu was 25. 437 μg / g,which was the lowest. The content of ergosterol in cordyceps cicadae from different areas varied from 120. 024 μg / g to 301. 652 μg / g. Conclusion There is difference among the contents of adenosine、cordycepin and ergosterol in Cordyceps cicadae from different areas; the method can be used to determine them in cordyceps cicadae,and to provide the reference for the quality evaluation of cordyceps cicadae.