MPPa-PDT抑制骨肉瘤MG-63细胞侵袭和迁移

MPPa-PDT inhibits invasion and migration of osteosarcoma MG-63 line cell

目的:探讨焦脱镁叶绿酸-a甲酯介导的光动力疗法(methyl ester pyropheophorbide-a mediated photodynamic therapy,MPPa-PDT)对人骨肉瘤MG-63细胞迁移及侵袭的影响及其可能机制。方法:采用CCK-8检测经MPPa-PDT处理后不同时间点的人骨肉瘤MG-63细胞增殖活性;划痕实验及Transwell实验检测细胞迁移、侵袭能力;Western blotting检测E-钙黏蛋白(E-cad)和基质金属蛋白酶(matrix metalloproteinase,MMP)-2、-9蛋白的表达水平。结果:MPPa-PDT处理MG-63细胞12、24及48 h后MPPa-PDT组细胞增殖能力明显较对照组、MPPa组和LED组细胞降低(均P<0.05),且MPPa-PDT组细胞的划痕愈合能力、迁移活力及侵袭能力也较这3组细胞显著下降(均P<0.05);MPPa-PDT组细胞E-cad的表达显著高于其他3组,MMP-2、MMP-9的表达则明显低于其他3组(均P<0.05)。结论:MPPa-PDT抑制人骨肉瘤MG-63细胞的侵袭和迁移能力;上调E-cad的表达,下调MMP-2、-9的表达可能是其作用机制之一。

Objective：To explore effect of methyl ester pyropheophorbide-a mediated photodynamic therapy （MPPa-PDT） on invasion and migration of human osteosarcoma MG-63 line cell and its possible mechanism.Methods：Using CCK8 assay,cytoactive of the MG-63 cell was detected at various time points of post-treatment with MMPa-PDT.Migration and invasion abilities of the MG-63 cell were respectively examined by Scratch and Transwell tests.Western blotting assay was used to check expression levels of E-cadherin （E-cad）,matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （MM-9） proteins in the MG-63 cell.Results：Mter treatment with MPPa-PDT,proliferation ability of the MG-63 cell at 12,24 and 48 h in MPPa-PDT group was significantly lower than those in control,MPPa and LED groups （all P ＜ 0.05）,and scratch healing ability,migration activity and invasion ability of the MG-63 cell in the MPPa-PDT group were also obviously more decline than those in the other three groups （all P ＜ 0.05）.Expression of E-cad protein in the MG-63 cell of the MPPa-PDT group was evidently higher than those in the MG-63 cell of the other three groups,while expressions of MMP-2 and MMP-9 proteins in the MG-63 cell of the MPPa-PDT group were lower than those in the MG-63 cell of the other three groups （all P ＜ 0.05）.Conclusion：MPPa-PDT could inhibit invasion and migration abilities of the human osteosarcoma MG-63 line cell,and could up-regulate expression of E-cad and down-regulate expressions of MMP-2 and MMP-9,which could be one of the mechanisms responsible for the above inhibition action.