不同抗凝剂对鲫鱼外周血的抗凝效果研究

Study on the Anticoagulation Effect of Different Anticoagulants on Peripheral Blood of Crucian Carp

[目的]研究不同抗凝剂对鲫鱼外周血的抗凝效果。[方法]以鲫鱼"中科3号"为研究对象,比较5种抗凝剂对鲫鱼外周血的抗凝效果,探讨其适宜抗凝剂品种和有效抗凝浓度。[结果]除了L-半胱氨酸在试验条件下不能作为有效抗凝剂外,其余4种抗凝剂延长血液凝固时间均在61.43 min以上。肝素钠、柠檬酸钠、草酸钾和EDTA的有效抗凝浓度分别为(0.80±0.17)、(1.42±0.14)、(1.17±0.14)和(2.33±0.58)mg/mL。显微镜下,肝素钠、柠檬酸钠、草酸钾和EDTA 4种抗凝剂均能较好地维持鲫鱼外周血细胞的形态。[结论]该研究结果可为鲫鱼外周血抗凝剂的应用提供参考。

[Objective] To study the anticoagulation effect of different anticoagulants on peripheral blood of crucian carp.[Method] Using crucian carp cultivar ＂Zhongke No.3＂ as research object,the anticoagulation effects of five kinds of anticoagulants on peripheral blood of crucian carp were compared.The suitable kind of anticoagulant and its effective concentration for peripheral blood of crucian carp were discussed.[Result]Under these test conditions,except for L-cysteine,other four kinds of anticoagulants could prolong the blood coagulation time more than61.43 min.The effective anticoagulation concentrations of heparin sodium,sodium citrate,potassium oxalate and EDTA were（0.80 ± 0.17）,（1.42 ±0.14）,（1.17 ±0.14） and（2.33 ± 0.58） mg/mL respectively.Under the microscope,the hemocyte morphology of peripheral blood in crucian carp treated with heparin sodium,sodium citrate,potassium oxalate and EDTA could be well maintained.[Conclusion] The research can provide references for the application of anticoagulants for peripheral blood of crucian carp.