基于Dy_2(MoO_4)_3-AuNPs复合纳米材料的葡萄糖生物传感器

Glucose Biosensor Based on Dy_2(MoO_4)_3-AuNPs Composite Nanomaterial

采用水热法合成了纳米材料钼酸镝[Dy_2(MoO_4)_3],并制备了Dy_2(MoO_4)_3-AuNPs复合材料,利用该复合材料固定葡萄糖氧化酶(GOD)构建了葡萄糖生物传感器.通过透射电子显微镜(TEM)、紫外-可见光谱(UV-Vis)和能谱分析(EDS)等手段对所制备的材料进行了表征,并利用电化学阻抗谱(EIS)和循环伏安(CV)曲线研究了该传感器的电化学性能.结果表明,Dy_2(MoO_4)_3-AuNPs复合材料具有较好的生物相容性,能增强固定化的GOD的生物活性,并促进GOD在电极表面的电子传递速率;该传感器在葡萄糖浓度为0.01~1.0 mmol/L范围内葡萄糖浓度与响应电流呈较好的线性关系,最低检出限为3.33μmol/L(S/N=3),该生物传感器还具有较好的稳定性和重现性.

Nanometer material of dysprosium molybdate[Dy2（MoO4）3] was hydrothermally synthsized and employed for the fabrication of glucose biosensor by immobilizing glucose oxidase（ GOD） on the Dy2（MoO4）3-Au NPs composite nanomaterial. Dy2（MoO4）3and Dy2（MoO4）3-Au NPs were characterized by transmission electron microscopy（ TEM）,UV-Vis spectrometry and energy-dispersive spectrometry（ EDS）. The electrochemical behavior of the biosensor was studied via the electrochemical impedance spectroscopy（ EIS） and cyclic voltammetry（ CV）. The experimental results indicated that Dy2（MoO4）3-Au NPs nanocomposite material presented good biocompatibility for GOD and could accelerate the electron transfer rate between the immobilized GOD and the electrode. The biosensor showed a good linear relationship in the concentration range of glucose from 0. 01 mmol/L to 1. 0 mmol/L with the lowest detection limit of 3. 33 μmol/L（ S/N = 3）. Additionally,the biosensor owned satisfying stability and reproducibility.