七氟醚麻醉对大鼠睡眠结构的影响

Effects of sevoflurane anesthesia on sleep architecture of rats

目的评价七氟醚麻醉对大鼠睡眠结构的影响。方法健康雄性SD大鼠16只，10～12周龄，体重300～350 g，采用随机数字表法，将其分为2组（n＝8）：对照组（C组）和七氟醚麻醉组（S组），均于腹腔埋入无线遥感植入子，用于监测肌电图和脑电图。S组吸入2.4%七氟醚与纯氧1.5 L/min 5.5 h，随后纯氧1.5 L/min洗脱0.5 h；C组吸入纯氧1.5 L/min 6 h。将大鼠放入睡眠监测观察箱，依据昼夜节律将麻醉后24 h（14：00至次日14：00）分为4个时间节段：L1（14：00至20：00）、D1（20：00至02：00）、D2（02：00至08：00）和L2（08：00至14：00）。采用Version 3.0 Neuroscore软件记录觉醒、非快动眼睡眠（NREM）和快动眼睡眠（REM）的总时间和次数以及每个时间段内觉醒、NREM和REM的时间。结果与C组比较，S组觉醒总时间缩短，REM总时间延长，NREM次数增加，L1和D1时段REM时间延长，D2时段觉醒时间缩短，NREM时间延长（P〈0.05），NREM总时间、REM次数、觉醒次数差异无统计学意义（P〉0.05）。结论七氟醚麻醉可改变大鼠睡眠结构的稳定性，增加REM，减少觉醒状态。

Objective To evaluate the effects of sevoflurane anesthesia on the sleep architecture of rats. Methods Sixteen pathogen-free healthy male Sprague-Dawley rats, aged 10-12 weeks, weighing 300-350 g, were divided into 2 groups （n= 8 each） using a random number table： control group （group C） and sevoflurane group （group S）. Each rat was implanted with a transmitter for recording electromyogram and electroencephalogram via telemetry. The rats were exposed to 2. 4% sevoflurane and pure oxygen 1.5 L/min for 5.5 h followed by 0. 5 h washout with pure oxygen in group S, and the rats were exposed to pure oxygen 1.5 L/min for 6 h in group C. Then the rats were taken into the sleep monitoring box, and the 24 h after anesthesia was divided into 4 time periods according to the circadian rhythm： L1 （14：00-20： 00）, D1 （20：00-02：00）, D2 （02：00-08：00） and L2 （08：00-14：00）. The total time spent on wakefulness, on non-rapid eye movement （NREM） sleep and on REM sleep, the number of wakefulness, NREM sleep and REM sleep, and the time spent on wakefulness, on NREM sleep and on REM sleep during each time period were recorded using Version 3.0 Neurosore software. Results Compared with group C, the total time spent on wakefulness was significantly shortened, the total time spent on REM sleep was prolonged, the number of NREM sleep was increased, the time spent on REM sleep in L1 and D1 time periods was prolonged, the time spent on wakefulness in D2 time period was shortened, the time spent on NREM sleep was prolonged （P〈0. 05）, and no significant change was found in the total time spent on NREM sleep or the number of REM sleep and wakefulness in group S （P〉0.05）. Conclusion Sevoflurane anesthesia can change the stability of sleep architecture, increase REM sleep and reduce wakefulness in rats.