基于荧光偏振免疫分析技术检测药茶中的黄曲霉素（英文）

A fluorescence polarization immunoassay for the detection of aflatoxins in herbal teas

本文旨在利用荧光偏振免疫(FPIA)分析技术检测被污染的药茶样品中的黄曲霉素。通过化学方法合成了荧光示踪物,并通过薄层色谱(TLC)和质谱(MS)进行检测。荧光偏振值通过偏振光进行检测。结果表明,黄曲霉素的FPIA检出限(LOD)为20ng·mL^(-1),半数抑制质量浓度(IC_(50))为371.80ng·mL^(-1),检测范围为92.76~252.32ng·mL^(-1)。与常规的高效液相检测方法相比较,FPIA检测方法具有快速,成本低等优点,可适用于药茶中黄曲霉素的高通量筛选。

A rapid fluorescence polarization immunoassay （FPIA） has been developed for the determination of aflatoxins in samples of naturally-contaminated herbal teas. The tracers were synthesized by chemical method and determined by thin layer chromatography （TLC） and mass spectroscopy （MS）. Fluorescence polarization was evaluated by the detection of polarized light. The results showed that the limit of detection （LOD） of FPIA for aflatoxins was 20 ng·mL^-1, the IC50 was 371.80 ng·mL^-1, and the linear range of the developed FPIA was 92.76-252.32 ng·mL^-1. Compared with conventional HPLC methods, the FPIA developed in this study has the advantages of short analysis time and low cost. This method may be suitable for highthroughput screening of aflatoxins in herbal teas.