非小细胞肺癌放疗抵抗细胞模型的建立与鉴定

Establishment and identification of radioresistant non-small cell lung cancer model

目的体外建立非小细胞肺癌A549放疗抵抗模型,为进一步研究放疗抵抗的机制和治疗提供实验基础。方法摸索接种细胞数量及诱导照射剂量;采用间歇照射诱导建立放疗抵抗细胞模型RA549;倒置显微镜观察细胞形态;克隆形成实验鉴定RA549的放疗抵抗性。结果照射后RA549细胞形态较亲本A549细胞变长、变大,但传代5代后又可恢复;RA549细胞的存活分数明显大于亲本细胞。结论间歇照射诱导可以成功建立稳定的、具有放疗抵抗性的肺癌细胞模型。

Objective To establish a radioresistant model of non-small-cell cancer A549,and to provide the experimental basis for further researchonradioresistance. Methods The cell number and the dose of radiation therapy were confirmed. The intermittent radiation were used to induce the radioresistant cell model. The morphology of cells were observed with an inverted phase contrast microscope. Colony formation was used to identify the radioresistance of the RA549 cell.Results RA549 cells were longer and bigger than A549 cells; surviving fraction of RA549 was significantly increased than A549. Conclusion Intermittent radiation can successfully induce the radioresistant cell model of lung cancer cells.