不同阳极电势驯化对于丁酸梭菌葡萄糖代谢影响研究

The glucose metabolism variation of clostridium butyricum during domestication with different anode potentials

构建丁酸棱菌Clostridium butyricum生物化学反应器,以研究不同驯化电势对其活性的影响.电池性能测试表明该菌具有良好的电活性.在该菌的生物电解池中,控制阳极电势(+0.6V、+0.2V和-0.2V,vs Ag/AgCl),电驯化菌株,结果显示该菌的电活性及葡萄糖代谢呈现出明显差异.0.6V和0.2V组的最大输出电流分别为1.8mA和1.2mA,而-0.2V组为0.02mA.高电势组内,乳酸、丁二酸和乙醇的含量逐渐减少,同时生成少量乙酸和丁酸.但-0.2V组则生成了大量丁酸(345mg/L)和生物质,伴随少量CO2生成(4%).实验结果表明高阳极电势促进生物膜的电极呼吸代谢,抑制其发酵代谢,从而表现出高生物电活性.

Clostridium butyricum was used for studying its glucose metabolism and bioelectrical activity with different anode potentials （＋0.6, ＋0.2 and -0.2 V, vs Ag/AgCl）. The cell performance test showed a good bioelectrical activity of the strain. During the domestication of the strain with three different anode potentials, the performances were quite different. At the end of domestication, the maximum output current of group 0.6 V and0.2 Vwere ca. 1.8 mA and 1.2 mA, while the group-0.2 V was only ca. 0.02 mA. The content of lactate,succinate and ethanol of different groups were decreased with the increasing of acetate and butyrate. Lots of butyrate（345 mg/L）and biomass were generated in group -0.2 V, while the content of CO2 （4%）was much less . The glucose metabolism analysis showed a transformation of metabolism from fermentation to anode-respiration with the bioelectrical activity.