摘要
调查规模化鸡场中产CMY-2大肠杆菌耐药基因与毒力基因的流行现状及共转移情况,对549株来源于鸡场粪样和苍蝇的大肠杆菌进行blaCMY-2PCR检测;K-B法检测耐药表型;PCR扩增19种相关耐药基因和14种毒力基因;接合试验和质粒复制子分型研究耐药基因与毒力基因的共转移.结果显示:6.0%(33/549)大肠杆菌呈CMY-2阳性,且均为多重耐药;检测到15种耐药基因和4种毒力基因,其中floR,blaTEM-1,sul2,sul1,traT,VagC检出率较高;63.6%产CMY-2大肠杆菌中blaCMY-2基因可通过lncA/C或lncI1质粒与耐药基因和(或)毒力基因共转移.本研究表明规模化鸡场粪样和苍蝇源产CMY-2大肠杆菌已成为耐药基因和毒力基因的重要储存库,携带二者质粒的转移导致耐药与毒力的传播扩散.
The co-transfer of resistance genes and virulence genes among CMY-2-producing- Escherichia coli from large-scale poultry farms in China was investigated. PCR was used to examine the presence of bla CMY-2 in 549 E. coli. Antimicrobial suseeptibilities were tested by diffusion methods. Gene detection and co-transfer were performed for 19 resistance genes and 4 virulence genes by PCR, conjugation experiment, and PBRT. Results showed 6. 0 %（33/549）isolates with multiple resistance carried bla CMY-2 gene. 15 resistance genes and 4 virulence genes were detected with high rate of floR, bla TEM-1, sul2, sul1, traT, and VagC. Co-transfer of resistance genes and virulence genes byplasmidsoflncA/Cor lncI1 was detected in 63.6 % CMY-2-positive-E. coli. This study demonstrated E. coli producing CMY-2 has become reservoirs of resistance genes and virulence genes and plasmids coharhoring these two factors lead to its dissemination among large-scale poultry farms.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第2期417-422,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家蛋鸡产业技术体系岗位科学家项目(CARS-41-K09)
国家重点基础研究计划(2013CB127200)
关键词
鸡场
大肠杆菌
CMY-2
耐药基因
毒力基因
共转移
Poultry farm
Escherichia coli
CMY-2
Resistance genes
Virulence gene
Co-transfer
