夏藤复方对肝火亢盛型高血压大鼠血压及血管活性物质的影响

Hypotensive Effects and Mechanism of Xiateng Compound Prescription in Treating Hypertensive Rats of Liver-Fire Exuberance

目的:观察夏藤复方对肝火亢盛型高血压大鼠血压及血管活性物质的影响。方法:将10只WKY大鼠作为正常组,并依据血压值差异将筛选的40只肝火亢盛型SHR大鼠随机分为:模型组、硝苯地平缓释片组、夏藤复方高剂量组、夏藤复方低剂量组,每组10只。14周龄开始灌胃给药,每天1次,连续4周。灌胃剂量相当于成人临床用药量6.25倍(人按照60 kg体质量计算)、灌胃体积10 m L·kg^(-1),夏藤复方高剂量组给予夏藤复方颗粒10.50 g·kg^(-1),夏藤复方低剂量组给予夏藤复方颗粒5.25 g·kg^(-1),硝苯地平缓释片组给予硝苯地平缓释片0.002 g·kg^(-1),正常组和模型组分别给予等量的去离子水。动态采集大鼠收缩压、行为学指标和宏观表征;酶联免疫吸附试验(ELISA)法检测大鼠给药4周后的血管紧张素Ⅱ(ANGⅡ)、去甲肾上腺素(NE)含量、内皮素(ET)、降钙素基因相关肽(CGRP)。结果:与模型组比较,夏藤复方高剂量组、夏藤复方低剂量组及硝苯地平缓释片组对收缩压均有不同程度地降低(P<0.05),其中硝苯地平缓释片组、夏藤复方高剂量组降压药效显著,且夏藤复方高剂量组的降压效果优于夏藤复方低剂量组(P<0.05);与模型组比较,夏藤复方高低剂量组旋转耐受时间、旷场实验之穿格次数、易激惹程度评分均有不同程度的改善(P<0.05),而硝苯地平缓释片组无明显变化(P>0.05);中药干预四周后,与模型组比较,夏藤复方高低剂量组大鼠血清中ANGⅡ、NE、ET、CGRP改善情况最好(P<0.05)。结论:夏藤复方可有效控制肝火亢盛证高血压大鼠收缩压升高,对其肝火亢盛证的症状有着显著改善,其作用机制初步认为与降低血清中ANGⅡ、NE、ET的含量,增加血清中CGRP的含量有关。

Objective： To observe the hypotensive effects and mechanism of Xiateng Compound Prescription in treating hypertensive rats of liver-fire exuberance. Methods： Ten WKY rats were selected as normal group and 40 SHR rats with liver-fire exuberance were selected according to difference of pressure value and randomized into model group,Nifedipine Sustained Release Tablet group,Xiateng Compound Prescription of high dosage group,Xiateng Compound Prescription of low dosage group,10 rats in each group. All rats were given intragastric administration of drugs from 14 weeks of age,once every day and for consecutive 4 weeks.The dosage of intragastric administration amounted to 6. 25 times of adult’s clinical dosage（ adult of 60 kg body mass）,and volume of intragastric administration was 10 m L·kg-1. Xiateng Compound Prescription of high dosage group were given Xiateng Compound Granules of 10. 50 g·kg-1,low dosage group 5. 25 g·kg-1,Nifedipine Sustained Release Tablet group given Nifedipine Sustained Release Tablet of 0. 002 g·kg-1; normal group and model group were given same amount of deionized water. The rats’ systolic pressure,behavior indicators and macroscopic signs were dynamically collected. The contents of ANG Ⅱ and NE,levels of ET and CGRP in rats were tested by ELISA after 4 weeks’ of administration. Results： Compared with model group,the systolic pressure in the Xiateng Compound Prescription of high dosage group,Xiateng Compound Prescription of low dosage group and Nifedipine Sustained Release Tablet group got declined to different degrees（ P 〈 0. 05）,and the hypotensive effects of Nifedipine Sustained Release Tablet group and Xiateng Compound Prescription of high dosage group were remarkable,and the effect of Xiateng Compound Prescription of high dosage group was superior to that in Xiateng Compound Prescription of low dosage group（ P 〈 0. 05）. Compared with model group,the rotation tolerance time,square-crossing times and irritating degree score in Xiateng Compound Prescription of high dosage group got improved to different degrees（ P 〈 0. 05）,but no obvious changes appeared in Nifedipine Sustained Release Tablet group（ P 〉 0. 05）. After 4 weeks of intervention,the improvements of ANGⅡ,NE,ET and CGRP in Xiateng Compound Prescription of high and low dosage groups were the best（ P 〈 0. 05）. Conclusion： Xiateng Compound Prescription can effectively prevent rise of the systolic pressure in rats with liver fire exuberance,which can significantly improve the symptoms of liver fire exuberance. The mechanism is associated with the decline of contents of ANGⅡ,NE,ET and the increase of content of CGPR in the serum.