紫叶李果实总黄酮抗氧化性能实验研究

Antioxidant Effect of Total Flavonoids in the Fruits of Pranus Cerasifera

目的考察紫叶李果实总黄酮(PCE)的抗氧化活性。方法采用紫外-可见分光光度法,在体外化学模拟条件下测定PCE的总还原能力,对1,1-二苯基-2-苦味肼基(1,1-Diphenyl-2-picrylhydrazy,DPPH)、超氧阴离子(O_2^-)、羟基自由基(·OH)、一氧化氮(NO)的清除作用及对脂质过氧化的抑制作用,研究PCE的抗氧化活性。结果 PCE在1,2,4,6,8 g/mL质量浓度下,吸光度逐渐增加(0.189±0.02,0.287±0.01,0.451±0.003,0.708±0.001,0.769±0.002),具有较强的还原能力,能明显地抑制·OH,O_2^-,DPPH,NO的生成;对照组、抗坏血酸组、PCE组(0.40,0.80,0.160 g/L)丙二醛(MDA)清除率分别为50.1%,19.6%,31.8%,45.9%,能有效抑制MDA的产生。结论 PCE具有较强的抗氧化活性,且呈明显的量效关系。

Objective To study the antioxidant effect of total flavonoids in the fruits of Pranus cerasifera（ PCE）. Methods The total reducing ability of PCE was determined by UV-Vis spectrophotometry. The total reduction ability of PCE,the effects of 1,1-Diphenyl-2-picrylhydrazy（ DPPH）,superoxide anion（O_2^-）,hydroxyl radical（·OH）,nitric oxide（NO） and the inhibition of lipid peroxidation were determined by the method of in vitro chemical simulation. The antioxidant activity of PCE was studied. Results The absorbance of PCE increased at the concentration of 1,2,4,6,8 g/mL（ 0. 189 ± 0. 02,0. 287 ± 0. 01,0. 451 ± 0. 003,0. 708 ± 0. 001,0. 769 ± 0. 002）,which had strong reducing ability and could significantly inhibit the generation of ·OH,O2-,DPPH,NO; The levels of malondialdehyde（ MDA）in the control group, ascorbic acid group and PCE group（ 0. 40,0. 80,0. 160 g/L） were 50. 1%,19. 6% and 31. 8%,45. 9%, respectively,showing the levels of MDA could effectively inhibited. Conclusion PCE has strong antioxidant effect and has obvious dose-effect relationship.