高效液相色谱法测定蓼子七中没食子酸含量

Content Determination of Gallic Acid in Antenoron Filiforme(Thunb.) by HPLC

目的建立测定蓼子七中没食子酸含量的高效液相色谱法。方法色谱柱采用InertSusTain C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(15∶85∶0.085),流速为1.0 mL/min,检测波长为270 nm,柱温为35℃。结果蓼子七中没食子酸质量浓度在0.141 5~0.830 5μg/mL范围内与峰面积线性关系良好(R^2=0.999 9),平均回收率为98.35%,RSD为0.60%(n=6)。结论该方法简便、快速,稳定性好,可用于蓼子七中没食子酸含量的测定。

Objective To establish an HPLC method for the content determination of gallic acid in Antenoron Filiforme（Thunb.）.Methods The chromatographic column was InertSusTain C_（18） column（ 250 mm × 4. 6 mm, 5 μm）, the mobile phase was methanol-water-phosphoric（15 ∶ 85 ∶ 0. 085） with a flow rate was 1. 0 mL/min,the detection wavelength was 270 nm and the column temperature was 35 ℃. Results The linear ranges of gallic acid in Polygonum seven was 0. 141 5-0. 830 5 μg/mL（R^2= 0. 999 9）. The average recovery was 98. 35% and RSD was 0. 60%. Conclusion The method is simple,rapid,has good stability,and can be used for the content determination of gallic acid in Antenoron Filiforme（Thunb.）.