玉米种子萌发响应淹水胁迫的定量蛋白质组学研究

Study on Quantitative Proteomics of Maize Seed Germination in Response to Waterlogging Stress

[目的]从蛋白质组水平上研究淹水胁迫对玉米种子萌发的影响,筛选重要蛋白质,阐明相关的代谢通路,为深入研究植物种子萌发过程中的耐淹机制提供新的思路。[方法]以玉米自交系196为试验材料,对种子萌发进行淹水胁迫72 h处理,利用TMT标记结合LC-MS/MS的定量蛋白质组学技术对蛋白质组成进行分析,对差异表达的蛋白质进行鉴定及功能分析。[结果]此次试验总共鉴定到1 456个蛋白,通过比较、筛选,鉴定到281个差异蛋白,其中244个上调蛋白,37个下调蛋白。通过对差异表达的蛋白质进行了GO功能富集分析和KEGG通路富集分析,发现这些差异蛋白大多参与了碳代谢、糖酵解过程、氨基酸代谢、抗逆与胁迫等过程。[结论]试验表明淹水胁迫抑制了玉米种子的萌发,通过不同代谢类型的动态变化和不同的信号转导来提高种子耐受性。

[Objective] To study the effects of waterlogging stress on maize seed germination from the level of proteome,screen important proteins and elucidate the related metabolic pathways,which provide new ideas for further study of flooding mechanism during seed germination.[Method]We chose maize inbred lines 196 as experimental materials to study the seeds germination under water stress for 72 hours.The protein composition was analyzed by TMT-labeled LC-MS/MS quantitative proteomics techniques.The differentially expressed proteins were analyzed by identification and functional analysis.[Result]A total of 1 456 proteins were identified in this trial.By comparison,we identified 281 differential proteins,of which 244 were up-regulated proteins and 37 down-regulated proteins.Through GO analysis and KEGG pathway analysis,we found those differential proteins were involved in the process of carbon metabolism,glycolysis,amino acid metabolism and stress resistance.[Conclusion]The experiment shows that waterlogging stress inhibits the germination of maize seed,and the seeds improve tolerance by dynamic changes of different metabolic types and different signal transduction.