蝴蝶花的化学成分研究

Chemical Constituents of Iris japonica

对鸢尾科鸢尾属植物蝴蝶花全草进行化学成分和生物活性研究。通过正相硅胶、Sephadex LH-20以及反相HPLC柱等等色谱方法对蝴蝶花的化学成分进行分离纯化,应用谱学技术鉴定化合物的结构。从蝴蝶花95%乙醇提取物中分离得到18个化合物,分别鉴定为:(+)-(7S,8R,7'E)-4-hydroxy-3,5'-dimethoxy-4',7-epoxy-8,3'-neolign-7'-ene-9,9'-diol 9'-ethyl ether(1)、丁香脂素(2)、erythro-guaiacylglycerol-β-O-4'-coniferylether(3)、threo-guaiacylglycerol-β-O-4'-coniferylether(4)、4-O-β-d-(6-O-vanilloylglucopyranosyl)vanillic acid(5)、4-[6-O-(4-hydroxy-3,5-dimethoxybenzoyl)-β-d-glucopyranosyloxy]-3-methoxybenzoic acid(6)、藏黄连新苷B(7)、belalloside B(8)、藏黄连新苷A(9)、茶叶花宁(10)、sakuranetin(11)、鼠李柠檬素(12)、鸢尾甲黄素B(13)、染料木素(14)、5,7,4'-三羟基-6,8-二甲氧基异黄酮(15)、野鸢尾苷元(16)、8-羟基鸢尾苷元(17)、3'-O-methylorobol(18)。其中,化合物1~18均为首次从该种植物中分离得到。化合物1、2、5、6、15为首次从该属植物中分离得到。对所分离得到的化合物进行了保肝、抗炎、抗氧化等体外药理活性筛选。

The whole plant of Iris japonica Thunb（Iridaceae） were investigated on their chemical constituents and bioac- tivities, eighteen compounds were isolated from an ethanolic extract from the whole plants of I. japonica by a combination of various chromatographic techniques including column chromatography over silica gel and Sephadex LH-20 and re- versed-phase HPLC. Their structures were determined by spectroscopic analysis including MS and NMR data. These compounds were identified as （ ＋ ） - （ 7S, 8R, 7＇ E） -4 -hydroxy-3,5＇-dimethoxy-4＇, 7 -epoxy-8,3 ＇-neolign-7 ＇-ene-9,9 ＇-di- ol9＇-ethyl ether （ 1 ）, syringaresinol （ 2 ）, erythro-guaiacylglycerol-β-O-4＇-coniferylether （3）, threo-guaiacylglyceml-β-O-4 ＇- coniferylether（ 4 ）, 4-O-β-d-（ 6-O-vanilloylglucopyranosyl ） vanillic acid （ 5 ）, 4-[ 6-0-（ 4-hydroxy-3, 5-dimethoxy- benzoyl） -β -d-glucopyranosyloxy ] -3-methoxybenzoic acid （6）, scroneoside B （7）, belalloside B （8）, scroneoside A （9）, apocynin （10）, 7-O-methylnaringenin （saknranetin） （ 11 ）, rhamnoeitrin （ 12 ）, iristectorigenin B （13）, genistein （ 14）, 5, 7,4＇-trihydroxy-6,8-dimethoxyisoflavone （ 15 ）, irigeni （ 16 ）, 8-hydroxytectrigenin （ 17 ）, 3＇-O-methylorobol （ 18 ）. Com- pounds 1-18 were obtained from this plant for the first time. Compounds 1,2,5,6,15 were obtained from this genus for the first time. The isolated compounds were screened for hepatoprotective, anti-inflammatory, antioxidant activities.