鼠尾草酸在人工体液中的稳定性考察

Stability of Carnosic Acid in Simulated Body Fluid

考察了鼠尾草酸(carnosic acid,CA)在人工体液中的稳定性及其降解产物。采用高效液相色谱法对鼠尾草酸在不同体液中的含量变化进行检测分析,同时使用制备型高效液相色谱对鼠尾草酸在人工体液中的降解产物进行分离。结果显示鼠尾草酸在人工胃液中未发生明显变化,而在人工肠液中发生了明显降解,主要产生4个降解产物,通过分离得到其中三个降解产物:迷迭香酚、表迷迭香酚和鼠尾草酚。实验表明碱性环境对鼠尾草酸的稳定性具有显著影响,制剂研究时必须考虑肠液对鼠尾草酸的降解作用。

This study investigated the stability of camosic acid（CA） in simulated body fluid using HPLC and preparative HPLC. The body fluid, including simulated gastric juice, simulated intestinal juice, blank simulated gastric juice and blank simulated intestinal juice ,was prepared according to ＂Chinese Pharmacopoeia＂. It was revealed that no significant changes of carnosic acid content was observed in simulated gastric juice and blank simulate juice ,while several degrada- tion products, such as rosmanol, epirosmanol, and camosol, were detected in simulated intestinal juice and blank simula- ted intestinal juice. Specifically, the pH value exerted a significant impact on the stability of CA in body fluid, which guided further development of CA.