蜂胶黄酮Pinobanksin-3-acetate对人胃癌SGC-7901细胞中HSPA6、GEM、FOS、RGS2、GADD45G基因表达的影响

Effects of Propolis Flavonoids Pinobanksin-3-acetate on the Expression of HSPA6,GEM,FOS,RGS2 and GADD45G Gene in Human Gastric Cancer Cell Line SGC-7901

考察蜂胶黄酮Pinobanksin-3-acetate(PB3A)对人胃癌SGC-7901细胞中相关基因表达的影响,用不同浓度(0、40、80μg/mL)的PB3A处理胃癌SGC-7901细胞24 h后提取总RNA,采用实时荧光定量PCR技术检测GEM、GADD45G、HSPA6、RGS2及FOS基因mRNA表达量,各个基因的DMSO对照组与40、80μg/mLPB3A处理组的ΔCt值进行比较。结果显示40μg/mLPB3A处理组与对照组比较HSPA6、GEM、FOS基因mRNA表达量明显提高(P<0.05),差异倍数分别为0.12(HSPA6)、2.17(GEM)、2.1(FOS),反而GADD45G、RGS2基因ΔCt无显著变化(P>0.05);80μg/mLPB3A处理组与对照组比较HSPA6基因mRNA表达量明显提高(P<0.05),其差异倍数为0.5,而其他基因mRNA表达水平无显著变化(P>0.05)。上述试验结果说明40μg/mLPB3A对人胃癌SGC-7901细胞中基因表达的影响比较明显;PB3A可能通过上调HSPA6、GEM、FOS基因表达而影响人胃癌SGC-7901细胞增殖。该研究结果为PB3A肿瘤治疗中的分子调控机制提供基础资料。

In order to investigate the effects of Propolis Flavonoids Pinobanksin-3-acetate （PB3A） on gene expression changes in human gastric cancer cell line SGC-7901 ,the cellline was treatedwith different concentrations of PB3A（0,40, 80 I.Lg/mL） ,and themRNAexpressionof ttSPA6, GEM,FOS,RGS2 andGADD45G geneswas detected by real-time quanti- tative PCR. The result showed that after treated the cell line with 40 μg/mL of PB3A, the expression of HSPA6, GEM and FOS genes were significantly increased （ P 〈 0.05 ）. The differential expression rates were 0. 12 （ HSPA6 ）, 2.17 （GEM） and 2.1 （FOS） respectively. However, theexpressionof GADD45Gand RGS2were not significantly increased（ P 〉 0.05 ）incomparison with untreated control group ;After treated the cell linewith 80μg/mL of PB3A, the expression of HSPA6 gene was significantly （ P 〈 0.05 ） increased, the differential expression rate of HSPA6 gene was 0.5, and the ex- pressionofGEM, FOS, RGS2 and GADD45 G geneswere not significantlyincreased （P 〉 0.05 ） by comparing to the control group. In conclusion, It was deduced that PB3A affected gastric cancer cell proliferation through regulating the expression of HSPA6, GEM and FOSgenes.