FTA-ABS和WB法对疑似梅毒患者血清特异性抗体检测复检结果分析

Evaluation of FTA-ABS and WB Assays for Specific Antibodies Detection in Patients with Suspected Syphilis

目的采用梅毒螺旋体抗体吸收实验(FTA-ABS)和免疫印迹技术(WB)法对临床疑似梅毒患者进行梅毒特异性抗体IgG和IgM检测。探讨该部分患者是否存在既往感染或隐性感染以及有无传染性。方法收集2012年3月-2013年5月于本院就诊的95例疑似梅毒患者血清(TPPA^+/TRUST^-或TPPA^+/TRUST^+滴度≤1∶4,既往无梅毒感染史也无相应的梅毒临床症状或体征,其配偶梅毒血清学结果阴性),采用FTA-ABS和WB法进行IgG和IgM特异性抗体检测。结果 FTA-ABS和WB法检测Ig G阳性病例分别为91例、90例;阳性率分别为95.79%和94.74%。两种方法分别与TPPA法比较,差异均无统计学意义(P>0.05)。FTA-ABS和WB法检测IgM阳性病例分别为16例和18例;两者检出阳性率分别为16.84%和18.95%。两种方法阳性检出率差异均无统计学意义(P>0.05)。结论 FTA-ABS和WB法IgG检测结果与TPPA符合率分别为95.79%和94.74%,证实疑似梅毒患者既往存在隐性梅毒感染史;在TPPA^+/TRUST^-或TPPA^+/TRUST^+滴度≤1∶4患者中,FTA-ABS和WB法IgM阳性检测结果分别为16例、18例,提示仍有少数患者可能存在近期感染。

Objective Serum anti-treponema pallidum IgG and IgM specific antibodies were detected to patients with suspected syphilis by Fluorescent treponemal antibody absorption test（FTA-ABS）and Western blot（WB）assays.To explore the possibility of those patients have the presence of previous infection or latent infection and contagiousness by specific syphilis serological tests.Methods A collection of syphilis serology screening data in our hospital inpatients and outpatients with suspected syphilis from March 2012 to May 2013.The syphilis serology test presented that TPPA＋/TRUST- or TPPA＋/TRUST＋ titer≤1：4.They denied any symtoms and cllincial manifestations before,at the same time their spouses are tested both negative.The data will be analyzed by FTA-ABS and WB assays of IgG and IgM specific antibodies from 95 patients.Results By FTA-ABS and WB assays,the positive rate for IgG were 95.79%, 94.74% in 91,90 cases,respectivetly while the consistent rate of two assays was 98.90%.Compared to TPPA there was no statistical difference between FTA-ABS and WB（P〉0.05）.There are 16,18 cases detected in IgM of two assays while the positive rate were 16.84%,18.95% respectively. The consistent rate of two assays was 97.89%.Conclusion Compared to TPPA,the coincidence rate of FTA-ABS and WB for TP-IgG were 95.79%, 94.74% respectivetly. It has confirmed that most of patients with suspected syphilis still have latent infection or prior infection.There are 16,18 cases were detected in TP-IgM of two assays.Few individuals have the possibility of recent infection or contagiousness in TPPA＋/TRUST-or TPPA＋/TRUST＋ titer ≤1：4.