结核患者外周血PBMC中T-bet和Eomes表达的检测

Detection of T-bet and Eomes in PBMC of patients with tuberculosis

目的初步探讨T-bet和Eomes的表达与结核病发生的关系。方法分析比较结核患者(TB)与健康对照(HC)、结核菌潜伏感染者(LTBI)以及细菌性肺炎患者(PN)外周血PBMC中T-bet和Eomes的m RNA表达水平,并观察结核患者在抗结核治疗前及抗结核治疗3、6、9和12个月后,外周血PBMC中T-bet和Eomes的m RNA表达水平的变化。结果结核患者(TB)外周血PBMC中T-bet和Eomes的m RNA表达水平显著低于健康对照(HC)、结核菌潜伏感染者(LTBI)以及细菌性肺炎患者(PN)(P<0.05)。同时,在抗结核治疗12个月后,T-bet的表达水平较治疗前显著升高(P<0.05),Eomes的表达水平较治疗前升高,但差异无统计学意义(P>0.05)。结论 T-bet和Eomes两个基因表达水平的下降可能与结核病的发生有关。

Objective To study the relationship between the T-bet/ Eomes and the occurrence of tuberculosis.Methods Comparative analysis of expression of PBMC T-bet/Eomes m RNA in tuberculosis（TB）,healthy controls（HC）,latent tuberculosis infection（LTBI） and bacterial pneumonia（PN）. Then,we observed the changes of T-bet/Eomes expressionbefore（0 m） and after 3 months,6 months,9 months and 12 months anti-tuberculosis therapy in the patients with tuberculosiscases. Results Expression of PBMC T-bet and Eomes in tuberculosis（TB） were significantly lower than those of healthycontrols（HC）,latent tuberculosis infection（LTBI） and bacterial pneumonia（PN）（P〈0.05）. At the same time,the expressionlevel of T-bet was significantly increased after anti-tuberculosis treatment for 12 months（P〈0.05）,Eomes also increased butthere was no significant difference（P〉0.05）. Conclusion The decreased expression of T-bet and Eomes may be related tothe occurrence of tuberculosis..