辣根过氧化物酶在双十六烷基磷酸膜中的直接电化学

Direct Electrochemistry of Horseradish Peroxidase in Surfactant Dihexadecylphosphate Film

利用表面活性剂双十六烷基磷酸（DHP）将辣根过氧化物酶（HRP）固定在EPG电极表面，研究了酶中Fe(（Ⅲ）／Fe（Ⅱ）电对与电极之间的直接电子传递过程以及酶催化双氧水还原过程，结果表明：（1）表面活性剂是一种固定酶的理想材料；（2）这种体系可能应用于构造第三代生物传感器．

The oxidation-reduction properties, relative to the Fe (Ⅲ) /Fe (Ⅱ) conversion, of horseradish peroxidase (HRP) in surfactant Dihexadecylphosphate films at EPG electrode have been investigated by using direct electrochemical methods. The electron transfer processes of HRP were described in the absence and in the presence of hydrogen peroxide. The results obtained are of potential value for basic and applied biochemistry and represent an opportunity to build the third generation of biosensors.