肥胖患者炎性单核细胞Caspase活性的研究

Caspase activity of pro-inflammatory monocytes in obese patients

目的:探讨外周血炎性单核细胞(CD14^+CD16^+)的Caspase活性与肥胖相关指标、空腹血糖(FBG)之间的相关性。方法:招募志愿者,测量身高、体质量、腰围,计算体质指数(BMI),血糖仪测定FBG。根据BMI分为3组:正常组、超重组、肥胖组。密度梯度离心提取外周血单个核细胞,流式细胞术检测CD14^+CD16^+单核细胞的数量以及细胞内Caspase活性。结果:肥胖组炎性单核细胞Caspase活性显著高于正常组和超重组,炎性单核细胞Caspase活性与BMI、腰围以及FBG正相关。结论:肥胖和高空腹血糖可能在炎性单核细胞Caspase活性升高中发挥作用。

Objective： To explore the correlation between caspase activity of pro-inflammatory monocytes （CD14＋CD16＋） and obese parameters, fast blood glucose （FBG）. Methods： Volunteers were recruited. Height, weight and waistline were measured and BMI was calculated. FBG was analyzed by glucometer. Volunteers were grouped based on BMI into normal group, overweight group, and obese group. Peripheral blood mononuclear cells were isolated by density gradient centrifugation. The number of CD14＋CD16＋ monocytes and the caspase activity in these cells were measured by flow cytometry. Results： Compared with normal and overweight groups, caspase activity of pro-inflammatory monocytes in obese group was significantly higher. Caspase activity of pro-inflammatory monocytes was positively correlated with BMI, waistline and FBG. Conclusion： Obesity and high fast blood glucose may play a role in the increase of caspase activity of pro-inflammatory monocytes.