癌胚抗原相关细胞黏附分子6在胆管癌中的表达及其意义

Expression and diagnostic value of biliary carcinoembryonic antigen-related cellular adhesion molecule 6 in cholangiocarcinoma

目的:检测胆管癌患者胆汁中癌胚抗原相关细胞黏附分子6(CEACAM6)的水平并判断其对胆管癌的诊断价值。方法:选取因胆管梗阻行手术治疗的患者63例,根据术后病理结果分为良性组(n=32)和胆管癌组(n=31)。于术中留取患者胆汁,应用ELISA法测定CEACAM6水平。应用Wilcoxon Rank Sum、线性回归、多元回归以及受试者工作特征曲线(ROC)评估CEACAM6对胆管癌的诊断价值。结果:与胆管癌组相比,良性组患者年龄更小,女性比例更高,CEACAM6水平更低(8.5 ng/mL vs 39 ng/mL,P≤0.001)。ROC曲线显示,当CEACAM6水平大于14 ng/m L时,其诊断胆管癌的敏感性为87.5%,特异性为69.1%。多元回归分析显示,碱性磷酸酶和支架数与CEACAM6相关。结论:胆管癌患者CEACAM6水平升高,可作为胆管癌的诊断指标。

Objective ：To detect the level of CEACAM6 in the bile of patients with biliary cancer and to explore）its diagnostic value for cholangiocarcinoma.Methods： Sixty-three patients were enrolled in this study and were divided into cholangiocarcinoma group（n=32） and benign group（n=31）.Bilewas collected during operation. The concentration of CEACAM6 was quantified by sandwich enzyme-linked immunosorbent assay （ELISA） and correlated with pathologic diagnosis. Diagnostic capability of CEACAM6 was evaluated by Wilcoxon rank-sum, linear regression, multiple regression, and receiver operating characteristic （ROC） curve analysis.Results：Patients in the benign group were younger, predominantly female, and had lower median biliary CEACAM6 levels than patients in the cholangiocarcinoma group （8.5 ng/mLvs. 39 ng/mL; P≤0.001）. ROC curve analysis determined CEACAM6 to be a positive predictor and a CEACAM6 level 〉14 ng/mL was associated with 87.5% sensitivity, and69.1% specificity. Multiple regression analysis suggested elevated alkaline phosphatase and the presence of biliary endoprosthesesmayinfluence CEACAM6 levels. Conclusion： Biliary CEACAM6 level may increase in patients with cholangiocarcinoma and can identify cholangiocarcinoma.