白藜芦醇对梗阻性黄疸大鼠肠道黏膜的保护作用

Protection Effect of Resveratrol on Intestine Mucosa in Rats with Obstructive Jaundice

目的:探讨白藜芦醇对梗阻性黄疸大鼠肠道黏膜的保护作用。方法:30只健康雄性SD大鼠随机分成SO+NS组、BDL+NS组和BDL+Res组,每组10只。BDL+NS组和BDL+Res组均结扎胆总管建立梗阻性黄疸大鼠模型,SO+NS组不结扎胆总管。BDL+Res组给予白藜芦醇(100mg/kg)灌胃,SO+NS组和BDL+NS组以生理盐水(10.0mL/kg)灌胃,1次/d。4周后取材,应用比色法检测回肠组织的MDA含量和SOD活性,HE染色法观察回肠组织的病理学改变,测定大鼠回肠黏膜厚度和绒毛长度。结果:BDL+NS组的肠黏膜明显变薄,绒毛萎缩变矮,其黏膜厚度和绒毛长度与SO+NS组和BDL+Res组比较差异有统计学意义(P<0.01);BDL+Res组的回肠组织病理性损伤明显减轻,其黏膜厚度和绒毛长度介于SO+NS组和BDL+NS组之间,与SO+NS组和BDL+NS组比较差异有统计学意义(P<0.01);BDL+NS组肠黏膜组织MDA含量明显高于SO+NS组,而SOD活性低于SO+NS组,与SO+NS组相比差异有统计学意义(P<0.01);给予Res治疗4周后大鼠肠道黏膜组织的MDA含量明显低于BDL+NS组(P<0.01),而SOD活性高于BDL+NS组(P<0.01)。结论:白藜芦醇可减轻肠黏膜的损伤程度,使肠黏膜厚度和绒毛长度得到修复,对梗阻性黄疸大鼠肠道黏膜有一定保护作用。

Objective：To explore the protective effect of resveratrol on intestine mucosa in rats with obstructive jaundice. Methods：30 healthy male Sprague-Dawley（SD）rats were randomly divided into 3 groups：SOt NS group （sham operation group）, BDL＋ NS group（bile duct ligation alone group） and BLD＋Res group （bile duct ligation plus Res feeding group）. Each group included 10 rats. Obstructive jaundice （OJ） was successfully established in BDL＋NS group and BLD＋Res group after bile duct ligation. For SO＋NS group,the choledochal duct was not ligated. BLD＋Res groups were given by oral administration with resveratrol （100 mg/kg）. SO＋NS groups and BDL＋NS groups were given by oral administration with 0.9% of NaCI liquid （10. 0 mL/kg）. The animals were killed 4 weeks after the experiment started. Colorimetric detection was made for intestinal tissue malondialdehyde（MDA） and superoxide dismutase（SOD） activity. HE stain method was used for morphological changes of ileum mucosa. The mucosal thickness and villus height d the ileum in rats were measured by Motic Med 6. 0. Results：In BDL＋NS group, HE stained sections of the ileum showed that intestinal mucosa of rats became thin evidently and intestinal villus had atrophy and became short. The thickness d intestinal mucosa and height of intestinal villus reduced more in BDL＋NS group than that in SO＋NS and BDL＋Res group（P〈0. 01）;In BDL＋Res group,pathological injury of the ileum tissues was obviously alleviated. The mucosal thickness and the height of intestinal villus in BDL＋Res group were between SO＋NS and BDL＋NS group. As compared with BDL＋ NS group,thickness of intestinal mucosa and height of intestinal villus were increased in BDL＋ Res group（P〈0. 01）,and as compared with SO＋NS group,the thickness of intestinal mucosa and the height of intestinal villus in BDL＋Res group were reduced（P〈0. 01）. Compared with SO＋NS group,the MDA content in intestinal mucosa of BDL＋NS group was significantly higher than that of SO＋NS group,but SOD of BDL＋NS group markedly reduced（P〈0. 01）. The above pathological changes were corrected after being treated with Res for 4 weeks and the content of MDA in intestinal mucosa significantly decreased compared with BDL＋ NS group（P〈0. 01）, but SOD increased（P〈0. 01）. Conclusion：Resveratrol can make the thickness of intestinal mucosa and height of intestinal villus to be repaired and it may play a beneficial role in alleviating intestinal mucosa injury.