胃黏膜癌变过程中RRM1和CDX2表达及相关性

Expression of RRM1,CDX2 in Carcinogenesis of Gastric Mucosa and their Correlation

目的:检测RRM1、CDX2在不同胃黏膜组织中的表达,探讨其临床意义。方法:收集胃癌90例,高级别上皮内瘤变、低级别上皮内瘤变、肠上皮化生、浅表性胃炎、正常胃黏膜各30例,共240例。采用S-P免疫组化方法对所有组织检测RRM1、CDX2表达,并进行分析。结果:(1)胃癌、高级别上皮内瘤变、低级别上皮内瘤变组RRM1的阳性表达率分别为86.7%、83.3%、83.3%,均高于浅表性胃炎组的66.7%(P<0.05)和肠上皮化生组的33.3%(P<0.01),浅表性胃炎组66.7%高于肠上皮化生组的33.3%(P<0.05);高级别上皮内瘤变、肠上皮化生、胃癌组的CDX2的阳性表达率分别为76.7%,63.3%、61.1%,均高于低级别上皮内瘤变组的33.3%(P<0.05)和浅表性胃炎组的0(P<0.01)。(2)210例不同胃黏膜病变中,RRM1与CDX2二者之间表达呈正相关(rs=0.352,P=0.000),90例胃癌中RRM1与CDX2二者之间呈正相关关系(rs=0.223,P=0.034)。结论:RRM1、CDX2表达均与胃癌的发生发展有关,二者可作为较为理想的肿瘤标志物,用于胃癌早期诊断的预警。

Objective： To detect the expression of RRM1 and CDX2, in different gastric mucosa lesions and esplore its clinical significance. Methods：Expression of RRM1 and CDX2 was detected in 6 groups of different gastric tissues 90 cases of gastric cancer,30 cases of high-grade intraepithelial neoplasia, 30 cases of low-grade intraepithelial neoplasia, 30 cases of intestinal metaplasia, 30 cases of superficial gastritis,30 cases of normal gastric mucosa using immunohistochemistry SP method by the correlation analysis. Results：（1） The positive expression rate of RRM1 in gastric cancer group, high-grade intraepithelial neoplasia group and low-grade intraepithelial neoplasia group were 86. 7% ,83. 3%and 83. 3%,respectively. Any one of the was higher than at superficial gastritis group which was 66. 7%（P〈0. 05） and at in- testinal metaplasia group which was 33. 3% （P〈0. 01）. The 66. 7% of superficial gastritis group was higher than 33. 3% of intestinal metaplasia group（P〈0. 05）;The positive expression rate of CDX2 in high-grade intraepithelial neoplasia group,intestinal metaplasia group and gastric cancer group were 76. 7%, 63. 3 % and 61. 1 %, respectively. Any one of the was higher than at low-grade intraepithelial neoplasia group which was 66. 7%（P〈0. 05） and superficial gastritis group which was 0（P〈0. 01）. （2）The expression of RRM1 in 210 cases of different Gastric Mucosa Lesions was positively correlated with CDX2（rs =0. 352 ,P=0. 000,P〈0. 01）. The expression of RRM1 In 90 cases of gastric cancer was positively correlated with CDX2（rs=0. 223,P=0. 034,P〈0. 05）. Conclusion：The expression of RRM1 and CDX2 is related to the development of gastric cancer. Hence,both of them can be used as ideal tumor markers for early diagnosis of gastric cancer.