卵巢切除对雌性小鼠脑皮质及海马区basigin表达的影响

Effects of oophorectomy on the expression of basigin in cortex and hippocampus of female mice

目的:通过检测basigin在雌性去卵巢小鼠脑皮质及海马组织中的表达变化,探讨体内雌激素水平与脑组织basigin表达的关系。方法:将28只小鼠随机分为手术组和假手术组,每组14只。手术组小鼠经背侧行卵巢切除术;假手术组不摘除卵巢。2个月后采用免疫组织化学法(immunohistochemistry,IHC)和western blot技术检测小鼠脑部皮质和海马basigin的表达变化。结果:IHC结果显示,相对于假手术组,手术组小鼠脑皮质和海马的CA1、CA3区basigin表达水平降低(P<0.05);Western blot结果表明,手术组小鼠脑皮质和海马basigin表达水平低于假手术组(P<0.05)。结论:脑皮质及海马basigin的低表达与体内雌激素水平下降有关。

Objective：To investigate the relationship between the level of estrogen in the body and the expression of basigin in brain tissue by detecting the expres- sion changes of basigin in oophorectomy mice cortex and hippocampus. Methods： The 28 mice were randomly divided into operated and sham - operated groups for each group was contained 14 mice. The operated group mice were removed ovaries via dorsal oophorectomy; sham -operated group mice were not removed ovaries. Immunohistochemistry （IHC） and western blot were applied to observe the expression of basigin in cortex and hippecampus after two months. Results： Compared with sham -operated group, the IHC data showed the expression levels of basigin were declined both in cortex and the CA1, CA3 areas in hippocampus （ P 〈 0.05 ） ; the western blot data indicated that the protein levels were reduced in operated mice cortex and hippocampus, respectively （ P 〈 0.05 ）. Condusion： Lower expression of basiginin cortex and hippocampus was related to the decline of estrogen levelin body.