不同固定液与染色法在病理切片中的应用比较

Comparison of different fixation and dyeing methods in pathological sections

目的:探讨不同固定液和苏木素染色法在病理诊断中应用价值。方法:分别应用10%中性甲醛和Fine Fix(FF)固定液以及不同Gill苏木素染色法对大鼠的肝、肾组织标本进行固定和染色,比较固定和染色效果。结果:FF固定液固定的组织形态结构完整;改良Gill苏木素液染色的病理切片,染色鲜亮,光镜下可见细胞核呈现蓝色,细胞质呈现红色,细胞核与细胞质染色对比清晰鲜明。结论:FF固定液固定的组织标本染色效果满意,无致癌和刺激性,废液处理方便;应用改良Gill苏木素液,成本降低,染色速度快,染色能力强,且环保性好。

Objective：To explore the application value of different kinds of fixative and haematoxylin staining method in pathologic diagnosis. Methods：The liver and kidney tissues of rat were fixed by 10% neutral formaldehyde and FF fixative, and stained by different Gill hematoxylin staining methods. The fixed and staining qualities were compared. Results： Morphological tissues fixed by FF fixative showed complete tissue struc- ture; Pathological sections stained by Gill hematoxylin were brightly dyed, and the nucleus appeared blue and cytoplasm red under light micro- scope, with clear and distinctive staining comparison between nucleus and cytoplasm. Conclnsions：The tissue sections fixed by FF fixative get bet- ter staining results without carcinogenicity and irritation, and the treatment of waste liquid is convenient; the improved Gill hematoxylin is low cost and fast dyed, with strong dyeing ability and good environmental protection.