摘要
目的建立金银花的HPLC对照指纹图谱,并在相同色谱条件下测定山银花的HPLC指纹图谱,通过计算相似度来区分山银花与金银花。方法色谱柱Phenomenex Luna 5μm C18(2)100 A(250 mm×4.6 mm,5μm);柱温40℃,以乙腈-0.5%磷酸溶液为流动相梯度洗脱,体积流量1 m L/min,检测波长为350 nm,相似度计算采取屏蔽大峰积分的方法。结果建立了金银花的HPLC对照指纹图谱,12批金银花相似度均在0.95以上,山银花相似度均小于0.80。结论 350nm下金银花的HPLC指纹图谱能有效区分金银花与山银花药材,屏蔽大峰积分的相似度评价方法比较准确地反映了金银花与山银花间化学成分的差异。
Objective To compare the differences between Lonicera Japonica Flos and Lonicera Flos by establishing HPLC fingerprint and calculating the similarity. Methods The columns was Phenomenex Luna 5 μm C18 (2) 100A, 250 mm×4.6 mm; The column temperature was 40℃. The mobile phase was acetonitrile-0.5% phosphoric acid, the flow rate was 1 mL/min, and the wavelength was 350 nm. Results HPLC fingerprint of Lonicera Japonica and similarity evaluation by screening large peak integration were established. The similarity of 12 batches of Lonicera Japonica Flos were all above 0.95, and four batches of Lonicera Flos were less than 0.80. Conclusion HPLC fingerprint profiles under 350 nm can reflex the differences between Lonicera Japonica Flos and Lonicera Flos effectively; Similarity evaluation by screening large peak integration shows the tiny differences of chemical component.
作者
刘亚楠
石典花
雷留成
LIU Ya-nan SHI Dian-hua LEI Liu-cheng(Henan Institute for Food and Drug Control, Zhengzhou 450003, China Shandong Academy of Chinese Medicine, Jinan 250014, China)
出处
《中草药》
CAS
CSCD
北大核心
2017年第4期773-776,共4页
Chinese Traditional and Herbal Drugs
关键词
金银花
山银花
HPLC指纹图谱
屏蔽大峰积分
相似度评价
Lonicera Japonica Flos
Lonicera Flos
HPLC-fingerprint profiles
screening large peak integration
similarity evaluation