一测多评法测定葛根药材中5种异黄酮类成分

Determination of five isoflavonoids in Pueraria Radix by QAMS

目的建立葛根药材中5种异黄酮类成分大豆苷元、大豆苷、葛根素、葛根素芹菜糖苷、葛根素-6″-O-木糖苷的一测多评法,验证该方法在葛根质量分析中应用的准确性及可行性。方法以葛根素为内标,分别建立葛根素与葛根素芹菜糖苷、葛根素-6″-O-木糖苷、大豆苷、大豆苷元的相对校正因子,计算葛根中葛根素芹菜糖苷、葛根素-6″-O-木糖苷、大豆苷、大豆苷元的量,实现一测多评。同时采用外标法测定葛根药材中5种异黄酮类成分的量,并比较计算值与实测值的差异,以验证一测多评法在测定中的科学性及可行性。结果各相对校正因子重复性良好,一测多评法测定结果与外标法测定结果无显著差异。结论以葛根素为内标同时测定葛根素芹菜糖苷、葛根素-6″-O-木糖苷、大豆苷、大豆苷元的一测多评法可用于葛根药材的定量分析。

Objective To establish a method for simultaneously determination of five isoflavones （daidzein, daidzin, puerarin, mirificin, and 6＂-O-xylosidepuerarin） in Pueraria Radix by single marker （QAMS）, which is feasible and accurate. Methods Puerarin was taken as internal standard substance to establish the relative correction factor （RCF） for quantitative analysis of multi-components with QAMS. Thus, the contents of mirificin, 6＂-O-xylosidepuerarin, daidzin, and daidzein were calculated. The contents in 10 batches of samples were determined by external standard method and QAMS. The scientificalness and feasibility of the methods were evaluated by comparison of the quantitative results between external standard method and QAMS. Results The reproducibility of RCF was perfect. The results calculated with QAMS were consistent with the results by the external standard method. Conclusion QAMS is accurate and feasible to evaluate the quality of Pueraria Radix.