小春花醋酸乙酯部位的化学成分研究

Chemical constituents from ethyl acetate extracts of Botrychium ternatum

目的研究小春花(阴地蕨Botrychium ternatum的全草)醋酸乙酯部位的化学成分。方法利用反相硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法等方法分离纯化,通过理化性质和波谱数据鉴定化合物的结构。结果从小春花的醋酸乙酯部位分离得到10个化合物,分别鉴定为卫矛醇(1)、30-nor-21β-hopan-22-one(2)、山柰酚-7-O-α-L-鼠李糖苷(3)、槲皮素-3-O-α-L-吡喃鼠李糖苷(4)、槲皮素-7-O-β-D-葡萄糖苷(5)、山柰酚-3-O-α-L-鼠李糖苷(6)、槲皮素-3-α-L-鼠李糖-7-O-α-D-葡萄糖苷(7)、山柰酚-3,7-O-α-L-二鼠李糖苷(8)、12-二十三酮(9)、β-胡萝卜苷(10)。结论化合物1、3~7和9为首次从阴地蕨属植物中分离得到,化合物2、10为首次从小春花中分离得到。

Objective To investigate the chemical constituents from ethyl acetate extracts of Botrychium ternatum. Methods The chemical constituents were isolated and purified by repeated silica gel chromatography, Sephadex LH-20 gel columns chromatography. Their structures were elucidated by analysis on the physicochemical properties and spectral data. Results Ten compounds were obtained from the ethyl acetate extracts of sceptridium and respectively elucidated as dulcite（1）, 30-nor-21β-hopan-22-one（2）, kaempferol-7-O-α-L-rhamnopyranoside（3）, quercetin-3-O-α-L-rhamnopyranoside（4）, quercetin-7-O-β-D-glucoside（5）, kaempferol-3-O-α-L-rhamnopyranoside（6）, quercetin-3-O-α-L-rhamnopyranoside-7-O-α-D-glucoside（7）, kaempferol-3,7-O-α-L-dirhamnoside（8）, tricosan-12-one（9）, and β-daucosterol（10）. Conclusion Compounds 1, 3—7, and 9 are obtained from the plants of Botrychium Sw. for the first time, compounds 2 and 10 are isolated from B. ternatum for the first time.