摘要
目的对我国不同产区的23份独蒜兰的遗传多样性进行分析,为后续资源利用奠定基础。方法利用ISSR分子标记技术和类平均法(UPGMA)对23份独蒜兰资源进行PCR扩增和数据分析。结果从100条ISSR引物中筛选出12条多态性较好的引物,在23份独蒜兰资源中扩增出86条DNA片段,其中多态性片段80条,多态位点百分率(PPB)为93.0%。23份独蒜兰资源被划分为2个类群,同一地区来源的大部分资源聚在同一类群或亚类群,也存在不同地区来源的资源间遗传关系较近的情况。结论 ISSR标记可为独蒜兰种质资源鉴定、遗传关系分析及品种培育提供一定依据。
Objective To analyze the genetic diversity and genetic relationship of 23 plant samples in Pleione bulbocodioides from different habitats. Methods The molecular marker technique of inter-sample sequence repeat（ISSR） and unweighted pair-group method with arithmetic means（UPGMA） were used to study the genetic diversity and cluster analysis of 23 samples. Results Twelve primers with diversity were sieved from 100 ISSR primers. DNA fragments（86） were amplified from 23 samples, and with 93.0% of polymorphic bands. In the cluster dendrogram, the studied samples were clustered into two groups, while the samples from the same region were in the same group and revealed the rule of geographical distribution in the tested materials. Conclusion The study provides the foundation for identification and development of plants in P. bulbocodioides and guide collection and evaluation of germplasm resources.
作者
张丽娜
桂阳
王沁
黄万兵
朱英
朱国胜
ZHANG Li-na GUI Yang WANG Qin HUANG Wan-bing ZHU Ying ZHU Guo-sheng(Institute of Modem Chinese Medicinal Materials, Guizhou Academy of Agricaltural Sciences, Guiyang 550006, China Biotechnology Institute of Guizhou Province, Guiyang 550006, China)
出处
《中草药》
CAS
CSCD
北大核心
2017年第5期979-984,共6页
Chinese Traditional and Herbal Drugs
基金
贵州省中药现代化科技产业研究开发专项项目[黔科合社字(2009)5036号
黔科合SY字(2014)3031-1号
黔科合中药字(2011)5039号
黔科合SY字(2014)3031-2号]
中央补助地方科技基础条件专项基金项目[黔科条中补地(2010)4002]
贵州省科技计划项目[黔科合院所创能(2010)4002]
贵州省农业科学院资助项目[黔农科合(创新基金)2012001号]
关键词
独蒜兰
ISSR
种质资源
遗传多样性
聚类分析
Pleione bulbocodioides（Franch
） Rolfe
inter-sample sequence repeat
germplasm resources
genetic diversity
cluster analysis
