地龙提取液中蛋白质的稳定性研究

Stability of Protein in Earthworm Extract

目的:对影响地龙提取液中蛋白质稳定性的因素进行研究。方法:通过考马斯亮蓝法建立了地龙提取液中蛋白质的含量测定方法,并考察了温度、pH值、无机盐及有机溶剂等因素对提取液中蛋白质稳定性的影响。结果:在30℃、pH值为7的条件下,提取液中蛋白质的含量最高,而加入无机盐或有机溶剂后蛋白质含量均有所降低。结论:提取液的最佳保存条件为30℃、pH值7,且提取过程中应尽量减少无机盐和有机溶剂的使用。

Objective： Factors influencing the stability of the protein in earthworm extract were researched in this article. Methods： Braford was adopted for determination of protein to observe the influences which temperature,pH,inorganic salt and organic solvents had on the stability of protein. Results： It showed that the protein was the stablest under 30℃ and pH = 7 and became weaker when inorganic salt and organic solvents were added.Conclusion： The optimal preservation condition for earthworm extract is 30℃ and pH = 7,and inorganic salt and organic solvents should be limited in the extraction process.