玄参多糖对正常生理小鼠和免疫低下状态小鼠免疫功能的影响

Effect of Polysaccharides of Radix scrophulariae on Immune Functions in Mice under Normal Physiological and Hypoimmunical State

目的:考察玄参多糖对正常生理小鼠和免疫低下状态小鼠免疫功能的影响。方法:将80只小鼠随机分为正常组,模型组,治疗高、中、低剂量组和生理高、中、低剂量组,每组10只。正常组和模型组小鼠ig蒸馏水(10 mL/kg),治疗高、中、低剂量组和生理高、中、低剂量组均按0.16、0.08、0.04 g/kg ig给药,每天1次,连续7 d;于给药第3天开始,模型组和治疗高、中、低剂量组小鼠分别ip环磷酰胺(100 mg/kg),连续4 d,复制免疫低下模型。采用碳廓清实验测定小鼠碳廓清指数和脏器(胸、脾)指数。另取80只小鼠分组、给药同上,采用绵羊红细胞致敏后测定其血清半数溶血值(HC_(50))。再另取80只小鼠分组、给药同上,采用1%二硝基氟苯法诱导小鼠迟发型变态反应测定其耳肿胀度,测定其血清中白细胞介素(IL)-2、IL-4、血清免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、γ-干扰素(IFN-γ)含量,并考察脾淋巴细胞的体外增殖情况。结果:高剂量玄参多糖可促进两种免疫状态下小鼠免疫器官及碳廓清指数的增长(P<0.05);增强小鼠迟发型变态反应的强度(HC_(50)值升高)(P<0.01)及改善模型小鼠血清溶血素含量的降低(P<0.01);促进脾淋巴细胞增殖(P<0.05)及升高血清中IL-2、IL-4、IgG、IgM、IFN-γ含量(P<0.05或P<0.01)。结论:玄参多糖可增强正常生理和环磷酰胺所致免疫低下两种状态下小鼠的免疫功能。

OBJECTIVE：To study the effect of polysaccharides of Radix scrophulariae on immune function in mice under normal physiological and hypoimmunical state. METHODS：80 mice were randomly divided into normal group,model group,treatment high-dose,medium-dose,low-dose groups and physiological high-dose,medium-dose,low-dose groups,10 in each group. Mice in normal group and model group were received distilled water（10 mL/kg） intragastrically,treatment high-dose,medium-dose,low-dose groups and physiological high-dose,medium-dose,low-dose groups were administrated 0.16,0.08,0.04 g/kg drug,ig,once a day,for 7 d. From the third day,mice in model group and treatment high-dose,medium-dose,low-dose groups were respectively received cyclophosphamide（100 mg/kg）,ip,for 4 d to induce hypoimmunical model. Carbon clearance test was adopted to determine the carbon clearance indexes and organs（chest,spleen）indexes of mice. Another 80 mice were grouped with the same administration,serum half hemolytic value（HC50）was determined after compressed Mianyang red blood cell sensitization. Then another 80 mice were grouped with the same administration,1% dinitrofluorobenzene method was used to induce delayed hypersensitivity in mice;its ear swelling was determined,as well as IL-2,IL-4,immunoglobulin G（IgG）,immunoglobulin M（IgM）,γ-interferon（IFN-γ）contents in serum,and the proliferation in vitro of splenic lymphocytes were detected. RESULTS：High-dose polysaccharides can promote the increasing of immune organ and carbon clearance indexes（P〈0.05）;enhance the intensity of delayed type hypersensitivity（HC50 increasing）（P〈0.01）and improve the decreasing of serum hemolysin in model mice（P〈0.01）;promote the splenic lymphocytes proliferation and increase IL-2,IL-4,IgG,IgM,IFN-γ contents in serum（P〈0.05 or P〈0.01）. CONCLUSIONS：Polysaccharides can enhance the immune function in mice under normal physiological and hypoimmunical state induced by cyclophosphamide.