葡萄籽原花青素对胆汁酸盐在结肠腺细胞Caco-2中转运的影响

Effects of Grape Seed Proanthocyanidin on the Transport of Bile Salts in Colon Glandular Cell Caco-2

目的:研究葡萄籽原花青素(GSP)对甘氨胆酸钠(GA)和牛磺胆酸钠(TA)在结肠腺细胞Caco-2中跨膜转运的影响。方法:利用Caco-2细胞模型,采用反相-高效液相色谱法测定细胞培养液中GA和TA的含量。试验分为GSP组、GA组、TA组、GSP+GA组和GSP+TA组,分别检测0、2、4、8 h从Transwell小室顶端(AP)侧透过Caco-2细胞向基底端(BL)侧转运GA和TA的透过量。结果:GA和TA检测浓度的线性范围均为0.05~1.2 mmol/L(R^2=0.999 9)。随着时间的延长,GA组和TA组BL侧GA和TA的透过量急剧增加;而加入GSP后,BL侧GA和TA的透过量明显较少,差异有统计学意义(P<0.01)。结论:GSP对GA和TA在Caco-2细胞中的跨膜转运具有抑制作用。

OBJECTIVE：To study the effects of grape seed proanthocyanidin（GSP）on the transmembrane transport of sodium glycocholate（GA） and sodium taurocholate（TA） in colon glandular cell Caco-2. METHODS：Caco-2 model was used,and RP-HPLC was conducted to determine the contents of GA and TA in cell culture medium. The test was divided into GSP group,GA group,TA group,GSP＋GA group and GSP＋TA group,the transport volumes of transporting GA and TA from Transwell apical（AP）side to basolateral（BL）side by Caco-2 cell at 0,2,4,8 h were detected,respectively. RESULTS：The linear ranges of GA and TA were 0.05-1.2 mmol/L（R-2=0.999 9）. With the time passing,transport volumes of GA and TA in BL site in GA group and TA group were sharply increased;while the transport volumes were obviously decreased after adding GSP,with statistical significance（P〈0.01）. CONCLUSIONS：GSP has inhibitory effect on the transmembrane transport of GA and TA in Caco-2 cell.