2,4,6-三硝基苯磺酸和葡聚糖硫酸钠诱导的大鼠肠炎模型比较

Comparison on the Model Rats with Colitis Induced by 2,4,6-three Nitrobenzene Sulfonic Acid and Dextran Sulfate Sodium

目的:比较2,4,6-三硝基苯磺酸(TNBS)和葡聚糖硫酸钠(DSS)诱导的两种肠炎大鼠模型。方法:SD大鼠随机分为正常对照组、TNBS模型组和DSS模型组,每组10只。TNBS模型组大鼠采用TNBS 50%乙醇溶液以100 mg/kg一次性灌肠法建立肠炎模型,DSS模型组大鼠采用4%DSS水溶液连续7 d自由饮用建立肠炎模型。观察各组大鼠一般情况,检测血清总胆固醇(TC)和三酰甘油(TG)含量、结肠长度及质量、结肠组织中髓过氧化物酶(MPO)活性,肉眼观察结肠大体外观形态,苏木精-伊红染色观察结肠病理学变化。结果:与正常对照组比较,TNBS模型组大鼠体质量明显减轻,结肠长度明显缩短、单位长度质量明显增加,粪便性状评分、血清TG含量、MPO活性、结肠大体外观评分和病理学评分均明显增加(P<0.05或P<0.01);DSS模型组大鼠体质量明显减轻,结肠长度明显缩短,粪便性状和结肠病理学评分均明显增加(P<0.05或P<0.01)。与DSS模型组比较,TNBS模型组大鼠结肠长度明显缩短、单位长度质量明显增加,血清TC、TG含量和结肠大体外观评分、病理学评分明显增加(P<0.05或P<0.01),组织病理学检查可见明显肠道溃疡与粘连。结论:DSS诱导肠炎模型大鼠粪便评分较TNBS诱导模型低,TNBS诱导肠炎模型大鼠在肠道组织损伤方面较DSS诱导模型更严重、恢复更慢。

OBJECTIVE：To compare the model rats with colitis induced by 2,4,6-three nitrobenzene sulfonic acid（TNBS）and dextran sulfate sodium（DSS）. METHODS：SD model rats were randomly divided into normal control group,TNBS model group and DSS model group,10 in each group. Rats in TNBS model group received 100 mg/kg disposable enema by TNBS 50%ethanol solution to induce colitis model,DSS model group received 4% DSS ethanol solution for 7 d by themselves to induce colitis model. The general conditions of rats were observed,total cholesterol（TC）and triglyceride（TG）contents,colon length and weight,myeloperoxidase（MPO）activity in colon tissue were detected,general appearance of colon was visually observed,and hematoxylin-eosin staining was used to observe the pathological changes. RESULTS：Compared with normal control group,body mass of rats in TNBS model group was reduced;colon length was shortened,and unit length quality was increased;feces character index,serum TG content,MPO activity,general appearance score and pathology score of colon were obviously increased（P〈0.05 or P〈0.01）. Body mass of rats in DSS model group was reduced;colon length was shortened;feces character and pathology score of colon were obviously increased（P〈0.05 or P〈0.01）. Compared with DSS model group,colon length of rats in TNBS model group was shortened;unit length quality was increased;serum TC,TG contents,general appearance score and pathology score of colon were obviously increased（P〈0.05 or P〈0.01）;histopathological examination showed obvious intestinal ulcers and adhesions. CONCLUSIONS：The feces score of model rat with colitis induced by DSS is lower than those induced by TNBS,while model rats induced by TNBS are more serious and slowly recover in terms of intestinal tissue injury than those induced by DSS.