均匀设计法优化藏药多血康提取工艺

Optimization of Extraction Technology for Tibetan Medicine Duoxuekang by Uniform Design

目的:优化多血康的提取工艺。方法:以红景天苷、没食子酸含量及出膏率的综合评分为指标,采用U6(63)均匀设计法优化多血康提取工艺中的料液比、乙醇体积分数及提取时间,再优化提取次数,并进行验证试验。结果:最优提取工艺为采用50%乙醇,料液比1∶14,浸泡1.5 h,回流提取1 h并重复2次;3次试验平均出膏率为50.18%,红景天苷和没食子酸含量分别为1.82、16.54 mg/g(RSD≤0.84%,n=3)。结论:优化的多血康提取工艺合理、简单、可行。

OBJECTIVE：To optimize the extraction technology of Duoxuekang. METHODS：Using comprehensive score of salidroside,gallic acid content and extraction yield as indexes,U6（63）uniform design was designed to optimize the liquid-solid ratio,ethanol volume fraction and extraction time of Duoxuekang,then optimize extraction times,and verification test was conducted. RESULTS：The optimal extraction technology was as follows as 50% ethanol,liquid-solid ratio of 1 ∶ 14,soaking time of 1.5h,reflux extraction for 1 h and repeated twice;the average extraction yield in 3 tests was 50.18%,contents of salidroside and gallic acid were 1.82 mg/g,16.54 mg/g（RSD≤0.84%,n=3）. CONCLUSIONS：The optimized extraction technology for Duoxuekang is reasonable,simple and feasible.