miR-98在哮喘患儿外周血单个核细胞中的表达及意义

The expression and clinical significance of miR-98 in peripheral blood mononuclear cells in asthmatic children

目的 探讨支气管哮喘患儿外周血单个核细胞（PBMCs）中miR-98的表达及其临床意义。方法 收集43例发作期和缓解期哮喘患儿，30例健康儿童（对照组）的外周血标本，分离PBMCs，采用实时定量PCR法检测PBMCs中miR-98及IL-13、IL-4 mRNA的相对表达量。结果 miR-98在哮喘发作期患儿PBMCs中的表达低于缓解期和对照组儿童，而IL-13、IL-4在哮喘发作期患儿PBMCs中的mRNA水平均高于缓解期和对照组，差异有统计学意义（P均〈0.01）；缓解期患儿和对照组间miR-98、IL-4 mRNA和IL-13 mRNA水平差异无统计学意义（P均〉0.05）。哮喘患儿发作期，miR-98水平与IL-4、IL-13 mRNA相对表达量均呈负相关（r＝－0.794、－0.804，P均〈0.001），IL-4和IL-13 mRNA相对表达量呈正相关（r＝0.853，P〈0.001）。结论 miR-98在哮喘发作期患儿PBMCs中表达降低，可能与小儿哮喘的发作相关。

Objective To explore the role of miR-98 in peripheral blood mononuclear cells （PBMC） in the pathogenesis and development of childhood asthma. Methods A total of 43 cases of asthmatic children and 30 cases of healthy controls were enrolled in the study. Peripheral blood mononuclear cells were isolated in both healthy subjects and asthmatic children in acute attack and remission stages. The expressions of miR-98 and interleukin-4 （ IL-4 ） and IL-13 mRNA were detected by real-time quantitative PCR. Results The miR-98 levels of asthmatic children in attack stage were significantly lower than those in remission stage and control group （P 〈 0.01 ）. The IL-4 and IL-13 mRNA levels of asthmatic children in attack stage were signif- icantly higher than those in remission stage and control group （P 〈0. 01 ）. There was no significant difference of miR-98, IL-4 and IL-13 mRNA between asthmatic children in remission stage and the controls （P 〉 0. 05）. Furthermore, a negative correlation was found between the expression of miR-98 and IL-5 （ r = -0. 794 ,P 〈0. 01 ） and between the expression of miR-98 and IL-13 mRNA （r = -0. 804, P 〈 0. 01 ） in asthmatic children in attack stage. A positive correlation was also found between IL-4 and IL-13 mRNA in asthmatic children in attack stage （ r = 0. 853, P 〈 0. 01 ）. Conclusion The expression of miR-98 decreased in asthmatic children, and miR-98 might be involved in the pathogenesis and development of asthma.