滇牡丹红斑病最适诱抗剂的筛选试验

The Study of the Optimal Inducers on Resistance to Cladosporium Red Spot of Paeonia delavayi

研究植物病害诱抗剂对滇牡丹红斑病的诱抗效应,以期为红斑病的防治提供一种新的途径。采用康壮素(Harpin)、苯并噻二唑(BTH)和水杨酸(SA)3种常见诱抗剂对滇牡丹红斑病进行了诱抗试验,分析了其发病指数、诱抗效果、生理等相关指标变化。结果表明:3种诱抗剂对滇牡丹红斑病诱抗效果显著,发病指数均显著下降,其中以BTH的诱抗效果最佳;并确定BTH的最佳使用浓度为100 mg/L,诱抗效果高达68.27%。在此浓度处理后,叶片病斑面积停止增长,边缘逐渐愈合,SAFR和MDA含量显著降低,可溶性蛋白、色素和相关酶系活力指标均显著增加,这些变化与流胶病的抗性变化密切相关。3种诱抗剂对滇牡丹红斑病原菌不产生毒杀作用,而是诱导植物系统获得性抗性(SAR),其作用机理有待进一步的研究。

In order to provide a new way to preventing the Cladosporium red spot, it was significant to study of the effect of plant disease inducers on resistance induction against red spot of Paeonia delavayi. 3 kinds of plant disease inducers that include Harpin, BTH and SA were been resistance test to red spot and the index of infection index, induced-resistance effects and related physiological and enzyme activity indexes were determined. The results showed that the 3 kinds of inducers had significant induced-resistance effects on the red spot and the infection index was dramatic decline. The optimal inducer was BTH and the best concentration was 100mg/L that inducedresistance effect to the red spot was to be 68.27%. The indexes of SAFR and MDA were dramatic decline, but the indexes of soluble protein, Pigment and related enzymes activity were obviously higher than those control processing, what were closely related with resistance induction to the red spot. Three kinds of inducers hadn′t toxic activity to the Cladosporium paeoniae and could lead P.delavayi to produce systemic acquired resistance （SAR） effectively, which mechanism would have to be further studied.