42个花生品种的SSR标记指纹图谱构建

Construction of Molecular Fingerprint for 42 Peanut Varieties Using SSR Markers

以不同地区的42个花生品种为材料,利用23对SSR引物进行分析。23对SSR引物在42个花生品种中共检测到92个等位变异,平均每对引物4个等位基因,平均PIC值为0.49。进一步分析表明,8对引物就可以将42个花生品种完全区分开,8对引物共产生40个等位变异,利用这40个等位变异构建了42个花生品种的DNA指纹图谱。聚类分析结果显示,在相似系数0.74处,可将42个供试品种分为4个类群。本研究结果为花生品种保护和育种实践提供了理论依据。

42 peanut varieties from different area of China were used as materials. The varieties were analyzed by using 23 pairs of polymorphic SSR primers. A total 92 alleles were amplified from 23 SSR loci. The average of alleles is 4, and the average of polymorphic information content （PIC） is 0.49. Further analysis showed that, only 8 pairs of primer can fully distinguish 42 peanut varieties. A total 40 alleles were amplified from these 8 pairs of primers. The fingerprint clustering analysis of 42 peanut varieties was constructed by 40 alleles, Cluster analysis revealed that 42 peanut varieties could be classified into four groups at a 0.74 genetic similarity coefficient. This result provide theoretical basis for peanut varieties protection and breeding improvement.