补中益气颗粒对脾气虚型变应性鼻炎大鼠血清IL-5及鼻黏膜IL-5 mRNA表达的影响

The Influence of Buzhong Yiqi Particle on IL-5 and the Expression of Nasal Mucosa IL-5 mRNA of rRats with Splenic Asthenic Allergic Rhinitis

目的通过观测补中益气颗粒对实验大鼠血清IL-5水平及鼻黏膜IL-5 m RNA表达水平的影响,探讨其治疗脾气虚型变应性鼻炎的作用机制。方法 (1)采用病症结合造模原理,在采用游泳力竭法、大黄法、饥饿法等多因素复合伤脾的方法造成大鼠脾气虚模型的基础上,用卵清蛋白为过敏原使大鼠致敏,建立脾气虚型变应性鼻炎大鼠模型。(2)以补气升阳,甘温除热的代表方药补中益气颗粒灌胃,创立脾气虚型变应性鼻炎大鼠中药组模型。(3)观察各模型组大鼠接受治疗后症状改变情况,用酶联免疫吸附法(ELISA)对各组血清中IL-5水平进行检测;用聚合酶链式反应(PCR)对各组鼻黏膜中IL-5 m RNA表达水平进行检测。结果与变应性鼻炎组及脾气虚型变应性鼻炎组相比,脾气虚型变应性鼻炎药物组症状改善明显,且血清IL-5水平及鼻黏膜IL-5 m RNA表达水平均明显降低(P<0.01)。结论补中益气颗粒治疗脾气虚型变应性鼻炎的作用机制之一是通过调节Th2细胞因子IL-5的表达,从而纠正Th2细胞因子的失衡状态,最终对脾气虚型变应性鼻炎产生治疗效果。

Objective Through observing the influence of Buzhong Yiqi particle on IL-5 and the expression of nasal mucosa IL-5 m RNA of rats with splenic asthenic allergic rhinitis, this paper discussed the mechanisms of splenic asthenic allergic rhinitis.Methodology（1） With combination of disease and symptom molding theory, methods of multi-factor impairment of the spleen like exhaustion method by swimming, rheum officinale and starvation method were employed to produce rats spleen-deficiency models,ovalbumin was used as an allergen to sensitize the rats and splenic asthenic allergic rhinitis rats models were constructed.（2） The representative traditional Chinese medicine of tonifying Qi and lifting yang as well as clearing heat with sweet-warm medicine-Buzhong Yiqi particle was used as the administration？by gavage to build rats models with splenic asthenic allergic rhinitis.（3） To observe symptom changes of each model and to test IL-5 levels with ELISA. Furthermore, PCR was used to test IL-5 m RNA expression of nasal mucosa. Result By comparing the group with allergic rhinitis with that with splenic asthenic allergic rhinitis, the latter one had more obvious improvement of symptoms and its IL-5 and the expression of IL-5 m RNA were significantly decreased（P0.01）. Conclusion One of the action mechanisms of Buzhong Yiqi particle on splenic asthenic allergic rhinitis is through adjusting the expression of Th2 cytokines IL-5 to rectify the imbalance states of Th2 cytokines so as to produce a therapeutic effect to splenic asthenic allergic rhinitis.