GPRC5A和SOCS3在大肠癌中的表达及临床意义

Expression and clinical significance of GPRC5A and SOCS3 in patients with colorectal carcinoma

目的:研究G蛋白偶联受体C家族5A(GPRC5A)和细胞因子信号转导抑制因子-3(SOCS3)在大肠癌中的表达及临床意义。方法:应用免疫组织化学(SP法)检测GPRC5A和SOCS3在45例大肠癌、25例大肠腺瘤及22例正常大肠黏膜组织中的表达。结果:1)GPRC5A在大肠癌组织中的表达(22.2%)明显低于腺瘤组织(52.0%,P<0.05),腺瘤组织中的表达明显低于正常大肠黏膜组织(81.8%,P<0.05)。GPRC5A的表达与有无淋巴结转移、Dukes分期、浸润深度密切相关(P<0.05)。2)SOCS3在大肠癌组织中的表达(24.4%)明显低于腺瘤组织(56.0%,P>0.01),腺瘤组织中的表达明显低于正常大肠黏膜组织(86.4%,P<0.05)。SOCS3的表达与肿瘤分化程度、浸润深度、Dukes分期、有无淋巴结转移有关(P<0.05)。3)GPRC5A与SOCS3在大肠癌组织中的表达呈正相关(r=0.503,P<0.05)。结论:GPRC5A和SOCS3的低表达参与了大肠癌的发生、发展,联合检测两蛋白可能作为临床判断大肠癌的恶性程度及预后的参考指标,并有望成为基因治疗的新靶点。

Objective： To study the expression and clinical significance of GPRC5A and SOCS3 in colorectal carcinoma. Methods： SP immunochemical method was performed to detect the expression of GPRC5A and SOCS3 in 45 cases of colorectal carcinoma, 25 cases of colorectal adenomas and 22 cases of normal colorectal tissues. Results： 1）Expression of GPRC5A in colorectal cancinoma tissue （22.2%） was significantly lower than that in adenomas tissue （52.0%, P〉0.05）.The Latter was significantly lower than that in normal colorectal tissue （81.8%, P〈0.05）. GPRC5A was closely related to lymph node metastasis, Duke＇ s stages and the deepness of invasion （P〈0. 05）. 2） Expression of SOCS3 in colorectal cancinoma tissue （24.4%） was significantly lower than that in adenomas tissue （56.0%, P〈0.01 ）. The Latter was significantly lower than that in normal colorectal tissue （86.4%, P〈0.05）. SOCS3 was closely related to pathological differentiation, the deepness of invasion, Duke＇ s stages and lymph node metastasis （P〈0.05）. 3）The expression of GPRC5A was positive correlated with SOCS3 （P〈0.05）. Conclusion： The reduced expressions of GPRC5A and SQCS3 may participate in the occurence and development of colorectal carcinorma, suggesting that GPRC5A and SOCS3 may act as biological markers for evaluating the malign degree, prognosis and therapeutic targets of colorectal carcinorma.